Effect of inhibitors of arachidonic acid metabolism on corneal reepithelialization in the rat

Experimental Eye Research
A G GuptaA D Proia

Abstract

We examined the ability of inhibitors of arachidonic acid metabolism to influence the rate of healing of organ-cultured rat corneas with 3-mm diameter central epithelial abrasions. In control corneas, and in the presence of the cyclooxygenase inhibitors indomethacin (1 microM), or flurbiprofen (1 microM), the defect was completely reepithelialized by 25 hr. In contrast, corneas cultured with the lipoxygenase inhibitors quercetin (100 microM), esculetin (100 microM), or baicalein (10 microM) or the dual cyclooxygenase/lipoxygenase inhibitors BW 775C (100 microM) or BW A540C (100 microM) had significantly delayed epithelial healing rates when compared with the controls; complete healing of the epithelial defects required 32.5-40 hr. Dose-response studies with esculetin and BW 755C demonstrated that the concentrations for 50% inhibition of reepithelialization (65.3 microM for esculetin, 39.6 microM for BW 755 C) were significantly greater than those for inhibition of 12-lipoxygenase activity (16.6 microM for esculetin, 21.1 microM for BW 755C), the major lipoxygenase activity in normal rat cornea. Addition of 12(S)-5,8,10,14- hydroxyeicosatetraenoic acid [12(S)-HETE, 0.01-10 microM], the main 12-lipoxygenase metabolite of arachido...Continue Reading

Citations

Nov 5, 1997·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·J S Hurst, H E Bazan
Mar 22, 2008·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Zaher A Radi, James A Render
Dec 8, 2009·Journal of Lipid Research·Sachidananda Kenchegowda, Haydee E P Bazan
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Jan 7, 2017·Experimental Biology and Medicine·Tina B McKay, Dimitrios Karamichos
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