Effect of inhibitors of trypsin-like proteolytic enzymes Bacillus cereus T spore germination.

Journal of Bacteriology
H BoschwitzW Troll

Abstract

The germination of Bacillus cereus T spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. Leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective inhibitors, whereas chymostatin, elastatinal, and pepstatin are inactive. A synthetic substrate of trypsin, tosyl-arginine-methyl ester, also inhibits germination. Its inhibitory effect decreases as a function of incubation time in the presence of spores and is abolished by previous hydrolysis with trypsin. Germinating, but not dormant, spore suspensions hydrolyze tosyl-arginine-methyl ester; its hydrolysis is insensitive to chloramphenicol, sulfhydryl reagents, and EDTA. A crude extract of germinated B. cereus spores contains a trypsin-like enzyme whose activity, as measured by hydrolysis of benzoyl-arginine p-nitroanilide, is sensitive to germination-inhibitory compounds such as leupeptin, tosyl-arginine-methyl ester, and tosyl-lysine-chloromethyl ketone. Spore suspensions exposed to the above inhibitors under germination conditions lose only part of their heat resistance and some 10 to 30% of their dipicolinic acid content. Part of the germinating spore population becomes "phase grey" under phase optics. Based on a study of the inh...Continue Reading

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Citations

Feb 19, 2002·Letters in Applied Microbiology·T HamoudaJ R Baker
Feb 14, 2007·The Journal of Biological Chemistry·Monique AkoachereErnesto Abel-Santos
Oct 6, 2010·Antimicrobial Agents and Chemotherapy·Zadkiel AlvarezErnesto Abel-Santos
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Apr 7, 2021·Biological Reviews of the Cambridge Philosophical Society·Jonathan AmponsahCalum R Wilson
Jan 6, 2016·Journal of Proteome Research·Linli ZhengChris G de Koster

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