PMID: 4612534Dec 1, 1974

Effect of initiation factor 3 binding on the 30S ribosomal subunits of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
C L Pon, C Gualerzi


Under certain conditions, initiation factor 3 (IF-3) can cause the release of aminoacyl-tRNA bound to 30S ribosomal subunits of E. coli. It is shown that this IF-3-induced aminoacyl-tRNA release cannot be attributed to either nucleolytic attack or competition between IF-3 and aminoacyl-tRNA for the same ribosomal binding site. It was found that the 30S-aminoacyl-tRNA-codon complexes formed in the absence of IF-3 are intrinsically different from those prepared in the presence of IF-3. In the absence of IF-3, the ribosomal binding of aminoacyl-tRNA is a virtually irreversible process, since the bound aminoacyl-tRNA can neither be spontaneously released upon dilution nor exchanged for unbound aminoacyl-tRNA. In the presence of IF-3, the binding of one molecule of IF-3 per 30S ribosome renders the binding of aminoacyl-tRNA reversible upon dilution and promotes exchange between bound and unbound aminoacyl-tRNA. It is suggested that this difference is due to a conformational transition of the 30S ribosomal subunit induced by the binding of IF-3. The possible implications of this finding in relation to the mechanism of action of IF-3 and its functional role in the cell are discussed.


Aug 23, 1972·Nature: New Biology·M Noll, H Noll
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S D BernalT Nakamoto
Jan 1, 1972·Molecular & General Genetics : MGG·C L PonC Gualerzi
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Apr 28, 1995·Journal of Molecular Biology·D MoazedHarry F Noller
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaClaudio O Gualerzi
Aug 14, 2001·The EMBO Journal·D PetrelliClaudio O Gualerzi
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·Claudio O GualerziC L Pon
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·Claudio O Gualerzi, Cynthia L Pon
Apr 27, 1979·Biochemical and Biophysical Research Communications·L GiriB Hess
Apr 16, 2008·Expert Opinion on Therapeutic Targets·Letizia BrandiC Gualerzi
Jan 25, 2007·Molecular Cell·Attilio FabbrettiC Gualerzi
Nov 26, 2005·Molecular Cell·Laura Lancaster, Harry F Noller
Jul 15, 1977·FEBS Letters·T Suryanarayana, A R Subramanian
Nov 1, 1975·European Journal of Biochemistry·H PontaM Schweiger
Dec 1, 1978·European Journal of Biochemistry·P ZiporiJ van Duin

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