PMID: 895556May 1, 1977

Effect of inoculum material on biosynthesis of proteolytic and amylolytic enzymes of Aspergillus tarricola

L A Aravina, V D Ponomareva


The effect of inoculation material on biosynthesis of proteolytic and amylolytic enzymes was studied with Aspergillus terricola H-20. The highest yield of proteolytic enzymes was found with the inoculation material obtained on oatmeal flakes with single shaking of the substrate. The highest number of viable conidia was detected by the sixth day of the growth; they favoured the synthesis of both proteases and amylases. The maximum yield of proteases was found when the density of inoculation was 6-10(6) conidia per 1 ml of the medium. The proteolytic activity of the fungus decreased after storage in a physiological solution at +4 degrees C for over 5 days; the amylolytic activity decreased if the conidia were kept under these conditions for two days.

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