PMID: 590653Dec 1, 1977

Effect of insulin on glycogen and protein synthesis in monolayer cultures of hepatocytes from normal and alloxan diabetic rats

Diabetologia
P BellemannD Mecke

Abstract

The effects of insulin on net glycogen synthesis and amino acid incorporation into protein were studied in cultured hepatocytes from adult normal and alloxan diabetic rats. Insulin stimulated glycogen synthesis in monolayer cells throughout a four day culture period and enhanced leucine incorporation into protein more effectively in normal cells with high glycogen levels than in cultured diabetic cells. These differences correlate well with the observed cellular ultrastructures which were maintained much better in the presence of insulin. Restoration of the morphological changes of alloxan diabetic hepatocytes to normal liver cell structures can be observed at any time during the culture period by giving insulin continuously.

References

Mar 24, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·D HegnerV Breuninger
Apr 1, 1976·Analytical Biochemistry·J R FryJ W Bridges
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M W ParizaV R Potter
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J KatzP A Wals
Dec 5, 1968·European Journal of Biochemistry·H de Wulf, H G Hers
Aug 6, 1973·Biochemical and Biophysical Research Communications·S R WagleL Sampson
Oct 23, 1974·Biochemical and Biophysical Research Communications·L J Crane, D L Miller
Nov 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·S R Wagle, W R Ingebretsen
Sep 1, 1972·The Biochemical Journal·D A HemsE A Taylor
Apr 1, 1974·Analytical Biochemistry·K L Roehrig, J B Allred
Apr 1, 1970·The Anatomical Record·C R Morgan, R A Jersild
May 22, 1970·Biochemical and Biophysical Research Communications·W GlinsmannE Hern

Related Concepts

Metazoa
Streptozotocin Diabetes
Glycogen
Insulin B Chain
Liver
Electron Microscopy
Protein Biosynthesis
Rats, Laboratory

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