Effect of Intense Pulsed Light Therapy on Tear Proteins and Lipids in Meibomian Gland Dysfunction

Journal of Ophthalmic & Vision Research
Salwa Abdelkawi AhmedAbd Elhakim Mohamed Safwat

Abstract

To assess the effect of a novel intense pulsed light (IPL) therapy on tear proteins and lipids in eyes with Meibomian gland dysfunction (MGD). Twenty-four eyes of 12 patients with MGD were recruited and received five overlapping flashes (565-1400 nm) directed at the lower eyelid. The IPL parameters include intensity: 2.5 to 6.5 J/cm2, voltage: 100 to 240 V, frequency: 50 to 60 Hz, input: 16 W, maximum optical energy: 23 J, pulse duration: <2.0 ms, and repetition time: 1-3.5 s. Tear samples were evaluated immediately before and 2 weeks after IPL therapy and included measurements of protein concentration, electrophoretic mobility by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, lipid profile assessments, and thin-layer chromatography (TLC) for phospholipids. Significant improvements were observed in tear protein concentrations and molecular weight after IPL therapy. The most pronounced effect was in the molecular weight of tear lysozyme, lactoferrin, and albumin. Tear lipids showed an improvement in the concentrations of total lipids, triglycerides, cholesterol, and phospholipids. On TLC, the tears in patients with MGD had significantly lower amounts of anionic phosphatidylethanolamine, phosphatidylinositol, an...Continue Reading

References

Aug 6, 2013·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Paweł PlakwiczEwa Monika Czochrowska

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Citations

Mar 18, 2020·The Cochrane Database of Systematic Reviews·Sharlotta CoteLaura E Downie
May 2, 2020·Photobiomodulation, Photomedicine, and Laser Surgery·Qingxia FanWei He
Jan 17, 2020·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Reiko Arita, Shima Fukuoka
Feb 23, 2021·Photobiomodulation, Photomedicine, and Laser Surgery·Lanting YangWei He
Aug 27, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Yilin SongEmmanuel Eric Pazo

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Methods Mentioned

BETA
electrophoresis

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