Abstract
Many tumors, including hepatocellular carcinomas (HCCs), resist Fas-mediated cell death, which is one of the effector mechanisms in the host's anti-tumor response; however, this resistance can be abolished by interferon-gamma (IFN-gamma). IFN-gamma may sensitize Fas-mediated cell death in several ways, but the exact mechanism in HCCs is uncertain. In this study, we thoroughly investigated the effect of IFN-gamma on the susceptibility of one human normal liver cell line and 12 HCC cell lines to Fas-mediated cell death. We also investigated the effect of IFN-gamma on the expression of various apoptosis-related genes such as the Fas/TNF-related genes, the bcl-2 family, and the caspase family of genes. Although most cell lines showed considerable constitutive expression of Fas, all tested cell lines resisted Fas-mediated cell death without IFN-gamma. When cells were pretreated with IFN-gamma, only three cell lines were made significantly susceptible to Fas-mediated cell death (SNU-354, SNU-387 and SNU-423); the other 10 cell lines were not affected. IFN-gamma increased the mRNA expression of Fas, TRAIL and caspase-1, and surface Fas was also increased. The strongly sensitized cell lines (SNU-354, SNU-387 and SNU-423) showed a parti...Continue Reading
Citations
Oct 6, 2011·Cancer Immunology, Immunotherapy : CII·Archana ThakurLawrence G Lum
Aug 8, 2002·Journal of Gastroenterology and Hepatology·Masanori MatsuiToshitaka Akatsuka
Jul 16, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Michael J Clemens
Oct 30, 2004·European Journal of Biochemistry·Sang-Youel ParkDai-Wu Seol
Feb 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Ja-Lok Ku, Jae-Gahb Park
Feb 16, 2002·Biochemical and Biophysical Research Communications·Sang-Youel ParkDai-Wu Seol
Apr 14, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·A LegrandG Feldmann
Oct 30, 2014·Cancer Immunology, Immunotherapy : CII·Xiaofeng WangMarianna Kulka
Dec 3, 2003·Oncogene·Su He WangJames R Baker
Dec 5, 2012·The Journal of International Medical Research·P ZhangY-F Jiang
Sep 29, 2007·Clinical and Experimental Immunology·N KoideT Yokochi
Mar 14, 2008·Clinical and Experimental Immunology·H-R KimJ Kim
Dec 31, 2004·Molecular Endocrinology·Myeong Goo YeoMi-Ock Lee
Nov 15, 2013·Clinical & Experimental Ophthalmology·Xiao Yang WangPaul R Healey
Feb 4, 2009·Journal of Cardiovascular Pharmacology·Victoria E A Stoneman, Martin R Bennett
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M A TaylorM Bennett
May 17, 2007·International Journal of Experimental Pathology·C T McCulloughD J Harrison