Effect of interferon on protein translation during growth stages of 3T3 cells

Archives of Biochemistry and Biophysics
R PetryshynR L Matts

Abstract

Interferons (IFNs) elicit a spectrum of biological responses from target cells, including inhibition of proliferation in several types of cells in vivo and in culture. The mechanism of action of IFN is complex and not fully understood. Previous evidence has indicated that part of the antiproliferative effect of IFN is due to modulation of protein translation. Here we report that there is a transient autocrine production of beta-interferon during specific periods of growth of mouse 3T3-F442A and 3T3-C2 cells. Treatment of preconfluent mouse 3T3-C2 cells with interferon reduced protein synthesis in these cells. This reduction began after 3 h of interferon treatment and was correlated with the appearance of phosphorylated double-stranded RNA dependent eIF-2 alpha kinase (PKR) measured in vitro. This inhibition of protein synthesis was associated with diminished exchange of GTP for GDP in the eLF-2.GDP complex. This diminished guanine nucleotide exchange activity was due to the inhibition of eukaryotic initiation factor eIF-2B, the factor required for the dissociation of GDP from eIF-2, and the formation of the functional eIF-2.GTP complex. The autocrine effect of IFN resulted in elevated PKR activity, increased phosphorylation of ...Continue Reading

Citations

Oct 23, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M J Clemens, A Elia
Dec 21, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Agnieszka Pindel, Anthony Sadler
Aug 19, 2007·Cytokine & Growth Factor Reviews·Stergios Moschos, John M Kirkwood
Sep 4, 2009·Journal of Cellular and Molecular Medicine·Rossen DonevJohannes Thome
Apr 27, 1999·The International Journal of Biochemistry & Cell Biology·R JagusG N Barber

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