Effect of intrafollicular indomethacin injection on gonadotropin surge-induced expression of select extracellular matrix degrading enzymes and their inhibitors in bovine preovulatory follicles

Reproduction : the Official Journal of the Society for the Study of Fertility
Qinglei LiGeorge W Smith

Abstract

A growing body of evidence supports an obligatory role for intrafollicular prostanoids in the mechanism of ovulation. However, the prostanoid-dependent mediators of the follicular extracellular matrix degradation required for ovulation are unknown. The objectives of this study were to determine the cellular compartment(s) in which the gonadotropin surge-induced regulation of select extracellular matrix degrading enzymes and their cognate inhibitors occurs in bovine preovulatory follicles, and to test whether such regulation is blocked by intrafollicular administration of the prostanoid synthesis and ovulation inhibitor, indomethacin (INDO). Follicular fluid prostaglandin E2 concentrations were elevated in diluent-treated follicles before ovulation (24 h after GnRH injection), but the increase was blocked in INDO-treated follicles. Real-time PCR analysis revealed the specific follicular cell types where gonadotropin surge-induced increases in mRNA abundance for members of the matrix metalloproteinase/tissue inhibitor of metalloproteinase and plasminogen activator families occurred. INDO treatment increased thecal cell mRNA for tissue inhibitor of metalloproteinase-4 and its protein abundance in the apex of preovulatory follicles...Continue Reading

References

Nov 1, 1985·Journal of Reproduction and Fertility·M De Silva, J J Reeves
Jul 1, 1972·Prostaglandins·A TsafririS A Lamprecht
Feb 1, 1997·Journal of Dairy Science·J R PursleyM C Wiltbank
Mar 17, 1999·Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association·A Tsafriri, R Reich
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
Apr 6, 2000·The Journal of Biological Chemistry·C MiyauraT Suda
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I TegederG Geisslinger
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 15, 2002·Reproduction : the Official Journal of the Society for the Study of Fertility·M P D DowG W Smith
Jun 5, 2002·Molecular and Cellular Endocrinology·Michael F SmithGeorge W Smith
Sep 26, 2002·Biology of Reproduction·Francisco GaytánJosé Eugenio Sánchez-Criado
Jan 7, 2004·Biochemical and Biophysical Research Communications·Lucio LagunasDavid Gius
May 4, 2004·Biochemical and Biophysical Research Communications·Hiromichi ItoEdward E Whang
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Qinglei LiGeorge W Smith
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Robert N WeinrebAlex Strongin
Nov 1, 1995·Theriogenology·J R PursleyM C Wiltbank

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Citations

Nov 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Qinglei LiGeorge W Smith
Sep 5, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Marcos H BarretaPaulo Bayard D Gonçalves
Sep 30, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·M GaytánF Gaytán
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·J Cuervo-Arango, J R Newcombe
May 13, 2014·Animal Biotechnology·Miguel A VelazquezHeiner Niemann
Nov 5, 2017·Reproductive Biology and Endocrinology : RB&E·Jacques G LussierKalidou Ndiaye
Jan 28, 2020·The Journal of Reproduction and Development·Rebecca LappAndreas Vernunft
Nov 20, 2020·Animal Cells and Systems·Patric S LundbergDiane M Duffy

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