Effect of intraluminal distension or ischemic strangulation obstruction of the equine jejunum on jejunal motilin receptors and binding of erythromycin lactobionate

American Journal of Veterinary Research
Judith B KoenigJonathan LaMarre

Abstract

To determine whether inflammation of the jejunum of horses decreases the number of motilin receptors and amounts of motilin receptor mRNA and alters erythromycin lactobionate binding affinity to the motilin receptor in jejunal tissues. Jejunal segments in 6 adult horses. Each horse was anesthetized, and a ventral median celiotomy was performed; 2 segments of jejunum underwent a sham operation, 2 segments underwent ischemic strangulation obstruction (ISO), and 2 segments underwent intraluminal distension (ILD). Treatments were maintained for 120 minutes. From each segment, full-thickness biopsy samples were collected and smooth-muscle homogenates were prepared. Affinity and distribution of motilin binding to these preparations were determined by use of iodine 125 (125I)-labeled synthetic porcine motilin. Via displacement experiments, competition between 125I-labeled motilin and erythromycin lactobionate for binding to motilin receptors in the different segments was investigated. A quantitative real-time PCR technique was used to assess motilin receptor mRNA content in the muscle preparations. Compared with the ISO or ILD segments, the number of motilin receptors was significantly higher in the sham-operated segments; ILD segment...Continue Reading

References

Oct 1, 1992·Alimentary Pharmacology & Therapeutics·J S JamesonM M Henry
Jan 1, 1991·Equine Veterinary Journal·J N King, E L Gerring
Jan 1, 1991·Peptides·I DepoortereG Vantrappen
Jan 1, 1988·Veterinary Surgery : VS·D AllenD E Tyler
Apr 1, 1988·The Veterinary Clinics of North America. Equine Practice·S B Adams
Nov 1, 1987·Veterinary Surgery : VS·J E ParkerH N Erb
Sep 1, 1986·Regulatory Peptides·V BormansG Vantrappen
Nov 1, 1993·Peptides·I DepoortereG Vantrappen
Dec 1, 1993·Gastroenterology·T L Peeters
Mar 1, 1996·Journal of Veterinary Internal Medicine·C B Navarre, A J Roussel
Jan 1, 1997·Peptides·Z Itoh
May 14, 1998·Australian Veterinary Journal·A J Dart, D R Hodgson
Apr 15, 2000·Peptides·P MillerP Poitras
Jan 9, 2001·The Anatomical Record·S M Ward, K M Sanders
Feb 13, 2001·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·I DepoortereT L Peeters
Feb 24, 2001·American Journal of Veterinary Research·R M DabareinerL L Donaldson
Jul 7, 2001·Journal of the American Veterinary Medical Association·A J RousselP C Rakestraw
Feb 15, 2002·American Journal of Veterinary Research·Jorge E NietoBrenna L Timmerman
Aug 24, 1965·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·W D PATON, H P RANG
Apr 24, 2004·Veterinary Surgery : VS·Linda M Van HoogmoedFaye A Harmon
May 11, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xuan-Zheng Shi, Sushil K Sarna
Dec 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jiean HuangKarnam S Murthy
Jan 29, 2005·The Journal of Pharmacology and Experimental Therapeutics·Bunzo MatsuuraLaurence J Miller
Mar 15, 2005·The Journal of Pharmacology and Experimental Therapeutics·Leen ThielemansTheo L Peeters

❮ Previous
Next ❯

Citations

Mar 18, 2009·Pediatric Surgery International·Asma RazzaqSalman Ali
Feb 11, 2009·The Veterinary Clinics of North America. Equine Practice·Eileen Sullivan Hackett, Diana M Hassel
May 12, 2021·Ocular Immunology and Inflammation·Richard R SadigKenneth G-J Ooi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.