Effect of intrathecally administered local anesthetics on protein phosphorylation in the spinal cord

Biochemical Pharmacology
R N NivarthiM Bansinath

Abstract

To elucidate the biochemical mechanisms of spinal anesthesia, we studied the effects of procaine and tetracaine on protein phosphorylation in the mouse spinal cord. Mice were injected intrathecally with either procaine, tetracaine (67 mM/approximately 2%, 10 microL, N = 5/drug), or saline (N = 4/group). Five minutes after injection, animals were killed with a guillotine, and the spinal cord was removed. The caudal 3-cm cord segment was homogenized and centrifuged, and an aliquot of the supernatant was used for phosphorylation assays. Calcium-dependent phosphorylation was initiated by incubating the samples in buffer containing [gamma-32P]ATP at 37 degrees for 30 min. The proteins were electrophoresed using slab gel and two-dimensional electrophoresis, and phosphorylated proteins were visualized by autoradiography. The data demonstrated that spinal anesthesia changes the phosphorylation state of five endogenous substrate proteins with apparent molecular masses of 130 (protein-a), 105 (protein-b), 55 (protein-c), 47 (protein-d), and 33 (protein-e) kDa. In two-dimensional electrophoresis, protein-a resolved into two proteins (a1 and a2). Analysis of variance of the densitometric data suggested a significant effect for the treatmen...Continue Reading

References

Nov 15, 1978·The Biochemical Journal·R F IrvineR M Dawson
Jan 1, 1991·Annals of the New York Academy of Sciences·J R TrudellW L Hubbell
Jul 1, 1990·Membrane Biochemistry·P P BabuM C Vemuri
Apr 1, 1990·Anesthesiology·J F Butterworth, G R Strichartz
Mar 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R K RalphP Schofield
Mar 1, 1990·The Journal of International Medical Research·K MikawaM Kusunoki
Jul 31, 1985·Biochemical and Biophysical Research Communications·Y UratsujiY Nishizuka
Nov 5, 1968·Biochimica Et Biophysica Acta·F A Henn, N Sperelakis
Jul 30, 1981·Biochemical and Biophysical Research Communications·T Tanaka, H Hidaka
Jan 1, 1980·British Journal of Pharmacology·W J TaylorJ M Young
Mar 30, 1995·Biochemical Pharmacology·G L Fraser, C J Fowler
Oct 1, 1994·Anesthesia and Analgesia·M H Kim-LeeJ S McDonald
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K T ShettyH C Pant
Apr 1, 1996·Annals of Emergency Medicine·D P Sklar
Feb 7, 1992·Science·B Hille

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Citations

Mar 18, 2009·Neurourology and Urodynamics·Simone D GueriosDale E Bjorling
Sep 23, 2008·The Journal of Experimental Biology·Lindsay A Jibb, Jeffrey G Richards

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