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Effect of ionic strength and ionic composition of assay buffers on the interaction of thyroxine with plasma proteins

The Journal of Endocrinology

Sep 1, 1975

Robert Lyndsay SutherlandM W Simpson-Morgan

PMID: 447

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Abstract

When plasma proteins are diluted with buffer the ionic strength and ionic composition of that buffer affects the interactions between thyroxine (T4) and its plasma protein-binding sites. Increases in phosphate, chloride or barbiturate ion concentration from 50 to 200 mmol/l caused a sig...read more

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Sodium Chloride
Thyroxine
Inorganic phosphate
Phosphate ion
Orthophosphate
Barbiturate [EPC]
Ampholytes
Total Thyroxine Measurement
Thyroxine Measurement
Thyroxine-Binding Proteins
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  • Effect of ionic strength and ionic composition of assay buffers on the interaction of thyroxine with plasma proteins

    The Journal of Endocrinology

    Sep 1, 1975

    Robert Lyndsay SutherlandM W Simpson-Morgan

    PMID: 447

    DOI:

    Abstract

    When plasma proteins are diluted with buffer the ionic strength and ionic composition of that buffer affects the interactions between thyroxine (T4) and its plasma protein-binding sites. Increases in phosphate, chloride or barbiturate ion concentration from 50 to 200 mmol/l caused a sig...read more

    Mentioned in this Paper

    Sodium Chloride
    Thyroxine
    Inorganic phosphate
    Phosphate ion
    Orthophosphate

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