PMID: 11316566Apr 24, 2001Paper

Effect of ionizing radiation on thymidine uptake, differentiation, and VEGFR2 receptor expression in endothelial cells: the role of VEGF(165)

International Journal of Radiation Oncology, Biology, Physics
P KermaniJ Fareh

Abstract

Late thrombosis of irradiated vascular segments may be the consequence of endothelial cell (EC) dysfunction after radiation therapy. We investigated the effects of beta ionizing radiation on human EC viability, thymidine uptake, and differentiation. Endothelial cells were exposed to (32)P-labeled DNA oligonucleotides in incremental doses of 2, 6, and 10 Gy. The modulation of the VEGFR2 receptor expression after irradiation and the overall potential radioprotective effect of VEGF(165) on these functions were assayed. A dose-dependent inhibitory effect of beta irradiation on ECs' thymidine uptake and differentiation was observed. EC viability, however, was not affected at levels of radiation up to 10 Gy. VEGF(165) proved to have a radioprotective effect as ECs' thymidine uptake, after radiation doses of 2, 6, and 10 Gy, was increased by 1.5-, 2-, and 4-fold, respectively, in the presence of 10 ng/ml of VEGF(165) (p < 0.05 vs. LacZ). This concentration of VEGF(165) also proved beneficial in maintaining cell differentiation at 16 h postirradiation when compared to controls. These biologic effects were in direct correlation with the upregulation of VEGFR2 receptor expression in irradiated ECs. beta irradiation interacts directly wit...Continue Reading

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Citations

May 5, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Takahiro HiguchiMarkus Schwaiger
Apr 17, 2008·Cancer Metastasis Reviews·Aaron P BrownKevin A Camphausen
Oct 8, 2003·International Journal of Radiation Oncology, Biology, Physics·Stefan Schultze-MosgauGerhard G Grabenbauer
Dec 13, 2006·Anti-cancer Drugs·Masahiko MiuraKengo Sakaguchi
Feb 15, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Lan WeiWeiguo Fu
Jan 3, 2006·American Journal of Ophthalmology·Richard F Spaide
Nov 12, 2015·Metabolomics : Official Journal of the Metabolomic Society·Tytus D MakAlbert J Fornace
Jul 21, 2005·International Journal of Radiation Oncology, Biology, Physics·Jing LiPaul M Harari
May 2, 2007·The Oncologist·Suresh Senan, Egbert F Smit

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