PMID: 61045Oct 5, 1976Paper

Effect of ionophores on intralysosomal pH

Biochimica Et Biophysica Acta
D J ReijngoudJ M Tager

Abstract

1. The effect of ionophores on the intralysosomal pH (as estimated from the distribution of a weak acid or base), on the distribution of 42K+ across the lysosomal membrane, and on the intralysosomal degradation of 125I-labelled bovine serum albumin has been studied. 2. Nigericin and X537A equilibrate both 42K+ and H+ across the lysosomal membrane. Gramicidin equilibrates H+ across the lysosomal membrane, this equilibration being more effective in a NaCl than in a KCl medium. Thus all three ionophores exhibit the same ion specificity as in other membranes. 3. The effect of the exchange-diffusion ionophores cannot be imitated by the combination of valinomycin with an uncoupler. Valinomycin by itself also has no effect. 4. X537A and nigericin inhibit the intralysosomal degradation of 125I-labelled albumin only when potassium is present. In a sucrose-containing medium no effect is found. Similar results were obtained with gramicidin. 5. These data suggest that the lysosomal membrane is impermeable to monovalent cations at 25 or 37 degrees C, and that the transport of protons is organised in such a way that electroneutrality is maintained.

References

Nov 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·K van Dam, E C Slater
Jun 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·G L Mandell
Aug 18, 1972·Biochimica Et Biophysica Acta·K D KussendragerM Gruber
Jan 24, 1973·Biochimica Et Biophysica Acta·D J Riejngoud, J M Tager

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Citations

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H A Chapman, J B Hibbs
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Kassidy K Huynh, Sergio Grinstein
Sep 1, 2011·The EMBO Journal·Jatta Huotari, Ari Helenius
Mar 21, 1978·Biochimica Et Biophysica Acta·R P CaseyJ M Tager
Dec 2, 1980·Biochimica Et Biophysica Acta·M HollemansJ M Tager
Jan 15, 1979·Biochemical and Biophysical Research Communications·P Dell'Antone
Aug 24, 2019·Scientific Reports·Nabab KhanXuesong Chen
Feb 28, 2009·Pediatric Research·Aviram Kogot-LevinGideon Bach
Sep 6, 2017·Frontiers in Cell and Developmental Biology·Li MaEric M Morrow
Jan 11, 1994·The American Journal of Physiology·A I PiquerasM L Zeidel
Jul 7, 2020·Cellular Signalling·Xuesong Chen, Jonathan D Geiger
Nov 14, 1977·Biochimica Et Biophysica Acta·D J Reijngoud, J M Tager

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