Effect of iron addition on mAb productivity and oxidative stress in Chinese hamster ovary culture.

Biotechnology Progress
Ryan J GrahamSeongkyu Yoon

Abstract

Trace metals play a critical role in the development of culture media used for the production of therapeutic proteins. Iron has been shown to enhance the productivity of monoclonal antibodies during Chinese hamster ovary (CHO) cell culture. However, the redox activity and pro-oxidant behavior of iron may also contribute toward the production of reactive oxygen species (ROS). In this work, we aim to clarify the influence of trace iron by examining the relationship between iron supplementation to culture media, mAb productivity and glycosylation, and oxidative stress interplay within the cell. Specifically, we assessed the impacts of iron supplementation on (a) mAb production and glycosylation; (b) mitochondria-generated free hydroxyl radicals (ROS); (c) the cells ability to store energy during oxidative phosphorylation; and (d) mitochondrial iron concentration. Upon the increase of iron at inoculation, CHO cells maintained a capacity to rebound from iron-induced viability lapses during exponential growth phase and improved mAb productivity and increased mAb galactosylation. Fluorescent labeling of the mitochondrial hydroxyl radical showed enhanced environments of oxidative stress upon iron supplementation. Additional labeling of...Continue Reading

References

Dec 1, 1995·Toxicology Letters·C C Winterbourn
May 10, 2000·The Journal of Nutrition·S R Powell
Apr 12, 2003·FEBS Letters·Barry Halliwell
Apr 15, 2004·Biochemical Pharmacology·François GaboriauGérard Lescoat
Jul 25, 2006·Current Biology : CB·Heidi M McBrideSylwia Wasiak
Dec 17, 2008·Annals of the New York Academy of Sciences·Anatoly A Starkov
Feb 12, 2010·Cellular and Molecular Life Sciences : CMLS·Qiongman Kong, Chien-Liang Glenn Lin
Feb 18, 2011·Biotechnology and Bioengineering·Michael J GramerPatrick H C van Berkel
Feb 22, 2011·Analytical and Bioanalytical Chemistry·Andrey V KuznetsovJakob Troppmair
Jul 27, 2011·Metallomics : Integrated Biometal Science·David J Eide
Mar 1, 2012·BMC Proceedings·A Rita CostaJoana Azeredo
Apr 15, 2014·Biotechnology and Bioengineering·Melissa M St AmandBabatunde A Ogunnaike
Aug 13, 2015·Glycobiology·Dietmar Reusch, Max L Tejada
Nov 13, 2015·Biotechnology Progress·Laurie B HazeltineAnli Ouyang
Mar 8, 2016·International Journal of Molecular Sciences·Hafiz Maher Ali ZeeshanHan-Jung Chae
Mar 27, 2016·Trends in Biotechnology·Sha ShaSeongkyu Yoon
Oct 23, 2016·Biotechnology Progress·Thomas K VilligerMiroslav Soos
Dec 3, 2016·Expert Review of Hematology·Bibbin T PaulSuzy V Torti
Mar 8, 2017·Biotechnology Progress·Jennifer KuschelewskiAline Zimmer
Jul 18, 2017·Analytical Biochemistry·Ljubava D ZorovaDmitry B Zorov
May 12, 2018·Applied Microbiology and Biotechnology·Anuja PrabhuMugdha Gadgil
Oct 21, 2018·Journal of Immunological Methods·Lina ChakrabartiPamela Hawley-Nelson
Aug 10, 2019·Cytotechnology·Berta Capella RocaMartin Clynes
Sep 7, 2019·Journal of Clinical Medicine·Estefania Burgos-MorónVíctor M Víctor
Dec 21, 2017·Antibodies·Devesh RadhakrishnanBabatunde A Ogunnaike
Dec 10, 2019·Biotechnology and Bioengineering·Valentine ChevallierLaetitia Malphettes
Dec 21, 2019·Applied Microbiology and Biotechnology·Ryan J GrahamChikkathur N Madhavarao
Jan 21, 2020·Biotechnology and Bioengineering·Michael W HandlogtenSanjeev Ahuja

❮ Previous
Next ❯

Citations

Jun 25, 2021·Biotechnology Progress·Duc HoangSeongkyu Yoon
Nov 10, 2021·Bioprocess and Biosystems Engineering·Amélie MahéPierre-Alain Girod

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Free Radical Biology & Medicine
Laura M Bystrom, Stefano Rivella
FEMS Microbiology Reviews
Simon C AndrewsFrancisco Rodríguez-Quiñones
© 2021 Meta ULC. All rights reserved