PMID: 2098700Oct 1, 1990

Effect of iron limitation on Bacteroides gingivalis

Oral Microbiology and Immunology
P K BaruaM E Neiders

Abstract

This study was undertaken to describe the effects of iron limitation on Bacteroides gingivalis. Four strains of B. gingivalis were grown in brain heart infusion broth, substituting protoporphyrin IX for hemin. Culture with protoporphyrin IX resulted in a loss of a 28 kDa membrane protein, but no decrease in growth. Iron-restricted cultural conditions for the growth of B. gingivalis were achieved using alpha/alpha'-dipyridyl, a ferrous iron chelator, at concentrations from 12.5 microM to 300 microM. Total suppression of bacterial growth for strain A7A1-28 and strain 381 was achieved at 200 microM alpha/alpha'-dipyridyl. At 300 microM alpha/alpha'-dipyridyl, strain W50 and Bowden 18/10 showed 100% and 80% suppression of growth, respectively. The ferric iron chelator Desferal did not show suppression of growth in concentrations up to 500 microM. The dipyridyl inhibition of cell growth for strain A7A1-28 could be reversed by adding excess ferrous ammonium sulphate but not by ferric nitrate. Iron regulation of proteolytic enzymes could not be demonstrated. Two new membrane proteins 42 kDa and 24 kDa are expressed with iron limitation, and the 45 kDa membrane protein was decreased with iron limitation.

References

Apr 15, 1979·The Biochemical Journal·H N ShahR A Williams
Mar 1, 1979·Journal of Bacteriology·R D Perry, R R Brubaker
Apr 1, 1979·Journal of Bacteriology·M J BjornB H Iglewski
Apr 1, 1978·Physiological Reviews·H N Munro, M C Linder
May 1, 1989·Journal of Periodontal Research·M E NeidersR J Genco
Apr 1, 1988·Infection and Immunity·K A PidcockT L Stull
Jan 1, 1988·Critical Reviews in Microbiology·S M Payne
Mar 1, 1988·Molecular Microbiology·A B Schryvers, L J Morris
Mar 1, 1988·Journal of Clinical Periodontology·A J van WinkelhoffJ de Graaff
Apr 1, 1987·Journal of Clinical Microbiology·D Grenier, D Mayrand
May 1, 1986·Infection and Immunity·A S McKeeP D Marsh
Apr 1, 1985·Infection and Immunity·D A Herrington, P F Sparling
Nov 12, 1970·The New England Journal of Medicine·U Muller-Eberhard
Jan 1, 1982·Annual Review of Microbiology·J B Neilands
Dec 1, 1983·The Journal of Applied Bacteriology·H N Shah, M D Collins
Jan 1, 1984·Physiological Reviews·E D Weinberg
Jan 1, 1981·Annual Review of Biochemistry·J B Neilands
Dec 1, 1982·The Journal of Infectious Diseases·A D O'BrienS B Formal
Jan 1, 1980·Infection and Immunity·D Mayrand, B C McBride
Nov 1, 1981·Reviews of Infectious Diseases·J J Bullen
Dec 1, 1982·Journal of Clinical Microbiology·J Slots, H S Reynolds

Citations

Apr 1, 1992·Journal of Periodontology·S S Socransky, A D Haffajee
Sep 1, 1991·Journal of Dental Research·E InoshitaSatoshi Shizukuishi
Apr 1, 1995·Journal of Dental Research·R C Page
Jan 18, 2006·Journal of Oral Science·Michiko Kiyama-KishikawaYoshimitsu Abiko
May 7, 2009·Future Microbiology·Rebecca E FitzpatrickRobert N Pike
May 1, 1991·Journal of Periodontal Research·S S Socransky, A D Haffajee
Jun 1, 1994·Periodontology 2000·A D Haffajee, S S Socransky
Jun 22, 1999·Infection and Immunity·M C Lynch, H K Kuramitsu
Feb 24, 2000·Journal of Periodontal Research·R C Page
May 15, 2002·FEMS Microbiology Letters·K-P Leung, Shawn P Folk
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakC A Genco
Nov 19, 2011·FEMS Microbiology Letters·Ji-Hoi MoonJin-Yong Lee
Jun 1, 1993·Periodontology 2000·K S Kornman, H Löe
Apr 3, 2003·Journal of Bacteriology·Mayuri ParamaesvaranCharles A Collyer
Dec 1, 1995·FEMS Microbiology Letters·A AmanoSatoshi Shizukuishi
Jun 26, 2003·Oral Microbiology and Immunology·L KesavaluJ L Ebersole

Related Concepts

Bacteroides
Desferal
Panhematin
Iron
Iron Chelating Agents
Cell Surface Proteins
Protoporphyrins

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