Effect of iron on expression of efflux pump (adeABC) and quorum sensing (luxI, luxR) genes in clinical isolates of Acinetobacter baumannii

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
Farzan ModarresiShahla Mansouri

Abstract

Resistance-nodulation-division efflux system (RND) adeABC contributes to intrinsic resistance to various drug classes in Acinetobacter baumannii. Similarly, quorum sensing (QS) plays an important role in the biofilm formation and pathogenicity of this bacterium. The aims of this study were to evaluate the influence of iron limitation on the expression of efflux pump (adeABC) genes and QS (luxI, luxR) system by relative quantitative real-time polymerase chain reaction (qRT-PCR). In addition, DNA sequence and phylogenetic relatedness of biofilm-associated protein (Bap) gene was also investigated. Sixty-five multidrug-resistant isolates of A. baumannii were recovered from ICU patients of three hospitals in Kerman, Iran. The isolates were highly resistant to at least 11 antibiotics (MIC ≥64 μg/mL); however, 87% and 89% were susceptible to colistin and tigecycline, respectively (MIC 0.05 μg/mL) (p ≤ 0.05). We detected the presence of RND efflux pump, QS, and bap genes with the frequencies of 92% (adeA), 61.5% (adeB), 84.6% (adeC), 80% (luxI), 61% (luxR), and 66% (bap), respectively. qRT-PCR analysis showed that in some isolates, expression of both adeABC and luxI/R was increased more than fourfold in the presence of low iron (20 μm)...Continue Reading

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Citations

Nov 2, 2016·Molecular & Cellular Proteomics : MCP·Takfarinas KentacheJulie Hardouin
Jul 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anabela BorgesManuel Simões
Apr 30, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Majid Taati MoghadamShahla Shahbazi
Jun 10, 2021·Human Vaccines & Immunotherapeutics·NorAziyah Mat RahimSazaly AbuBakar
Apr 28, 2021·Antimicrobial Agents and Chemotherapy·Vanessa Kornelsen, Ayush Kumar

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