Effect of isothiocyanates on nuclear accumulation of NF-kappaB, Nrf2, and thioredoxin in caco-2 cells

Journal of Agricultural and Food Chemistry
Jana JakubíkováYongping Bao

Abstract

Early effects (only 1 h of exposure) of three isothiocyanates (benzyl, phenylethyl, and sulforaphane) on nuclear accumulation of thioredoxin, APE/Ref-1, and transcription factors NF-kappaB and Nrf2, as well as production of reactive oxygen species (ROS) and reduced glutathione levels were examined in human adenocarcinoma Caco-2 cells. Nuclear increase of NF-kappaB, Nrf2, and thioredoxin contents was observed in all isothiocyanate-treated cells, whereas the nuclear Ref-1 and cytoplasmic Keap1 contents were not changed. Sulforaphane was the most potent inducer of Nrf2 nuclear accumulation (10 microM, 1.9-fold) and NF-kappaB nuclear accumulation at higher concentration (25 microM, 6.3-fold). In contrast, benzyl isothiocyanate induced more thioredoxin nuclear accumulation (10 microM, 2.9-fold), increased production of ROS, and gave the greatest induction of thioredoxin reductase 1 mRNA (10 microM, 10.2-fold), whereas phenylethyl isothiocyanate was more potent in the depletion of reduced glutathione levels. These results show that different individual isothiocyanates may possess some different activities in nuclear accumulation of thioredoxin, NF-kappaB, Nrf2, and production of ROS.

References

Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·R Venugopal, A K Jaiswal
May 18, 1999·Biochemical and Biophysical Research Communications·S HashimotoT Horie
Sep 30, 2000·European Journal of Biochemistry·E S Arnér, A Holmgren
Sep 30, 2000·European Journal of Biochemistry·K BeckerS Müller
Oct 20, 2000·Analytical Biochemistry·H KamencicB H Juurlink
Mar 10, 2001·Recent Progress in Hormone Research·J AvruchX F Zhang
Jun 19, 2001·The Journal of Biological Chemistry·E HeissC Gerhäuser
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Makoto KobayashiMasayuki Yamamoto
Sep 7, 2002·Antioxidants & Redox Signaling·Shugo UedaJunji Yodoi
May 14, 2003·The Journal of Nutritional Biochemistry·Korry J. HintzeElizabeth H. Jeffery
Aug 29, 2003·Cellular and Molecular Life Sciences : CMLS·P RoseC N Ong
Sep 11, 2003·FEBS Letters·Mariarosaria NapolitanoRoberto Rivabene
Sep 19, 2003·Trends in Biochemical Sciences·Paola Chiarugi, Paolo Cirri
Sep 19, 2003·Biochemical and Biophysical Research Communications·Tetyana KhomenkoSandor Szabo
Dec 24, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Walter H WatsonJames P Kehrer
Jul 30, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jason M HansenDean P Jones
Aug 18, 2004·Cancer Research·Melinda C MyzakRoderick H Dashwood
Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Peter RoseMatthew Whiteman
Oct 12, 2004·Experimental Cell Research·Juliet M TaylorRocco C Iannello
Dec 8, 2004·Archives of Biochemistry and Biophysics·Yasutake KatohMasayuki Yamamoto
Jan 5, 2005·Archives of Biochemistry and Biophysics·Izumi NakashimaHaruhiko Suzuki
Jul 7, 2005·Journal of Agricultural and Food Chemistry·Korry J HintzeJohn W Finley

❮ Previous
Next ❯

Citations

Jul 11, 2006·Apoptosis : an International Journal on Programmed Cell Death·Juraj BodoJan Sedlak
Mar 20, 2012·The British Journal of Nutrition·Eliz WarwickYongping Bao
May 22, 2010·Antioxidants & Redox Signaling·Ryan Holland, James C Fishbein
Nov 8, 2014·Microbiology·Virginie DufourChristine Baysse
Jun 11, 2015·Antioxidants & Redox Signaling·Marcus CebulaElias S J Arnér
Jul 22, 2010·Nature Reviews. Urology·Vasundara Venkateswaran, Laurence H Klotz
Apr 25, 2012·Molecular and Cellular Biochemistry·Katarzyna LubelskaKatarzyna Wiktorska
Dec 15, 2012·Pharmacological Reports : PR·Katarzyna LubelskaKatarzyna Wiktorska
Aug 17, 2016·Oncotarget·Sona HudecovaOlga Krizanova
Jun 23, 2011·Journal of Cellular and Molecular Medicine·Anika Eva WagnerGerald Rimbach
Oct 30, 2020·Antioxidants & Redox Signaling·Junmin ZhangJianguo Fang
Aug 30, 2008·Chemical Research in Toxicology·Ryan HollandJames C Fishbein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.