Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E2 Generation in LPS-stimulated RAW264.7 Cells

Immune Network
Yu Yeon JungDong Ju Son

Abstract

Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE2) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE2 generation via suppression of iNOS and COX-2 expression.

References

Feb 28, 2002·Trends in Immunology·Sarah G HarrisRichard P Phipps
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Nagatoshi Fujiwara, Kazuo Kobayashi
Oct 5, 2010·Planta medica·Mi-Ran ChaShi Yong Ryu
Jan 5, 2011·Cell Metabolism·Rebecca G BakerSankar Ghosh
Apr 22, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Emanuela Ricciotti, Garret A FitzGerald
Apr 17, 2013·International Journal of Molecular Medicine·Sung-Bae KimDong-Yeul Kwon
Oct 3, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hwa-Young LeeHyung-Ryong Kim
Nov 8, 2014·Asia Pacific Allergy·Tae-Hyeong LeeChoon-Sik Park
Nov 10, 2014·Immunologic Research·Carlo SelmiCarlo A Sciré
Jun 2, 2015·Trends in Pharmacological Sciences·Yasuko Iwakiri, Moon Young Kim
Jun 25, 2015·BMC Complementary and Alternative Medicine·Yong-Deok JeonSeung-Heon Hong
Sep 6, 2015·Bioorganic & Medicinal Chemistry Letters·SeonJu ParkSeung Hyun Kim

❮ Previous
Next ❯

Citations

Apr 4, 2017·The American Journal of Chinese Medicine·Jae Sik YuJae Youl Cho
Mar 10, 2017·Journal of Ethnopharmacology·Woo Seok YangJae Youl Cho
Sep 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chinreddy Subramanyam ReddyAnd Yeon Bok Kim
Apr 6, 2018·Journal of Medicinal Food·Shan HuangBin Li
May 16, 2018·International Journal of Molecular Sciences·Sang Yun HanJae Youl Cho
Apr 3, 2019·International Journal of Molecular Sciences·Eunji KimJae Youl Cho
Aug 24, 2017·The American Journal of Chinese Medicine·Woo Seok YangJae Youl Cho
Jul 7, 2017·Mediators of Inflammation·Jae Gwang ParkJae Youl Cho
Feb 22, 2017·Scientific Reports·Muhammad Jahangir HossenJae Youl Cho
Jul 15, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sulgi YooJae Youl Cho
Jun 24, 2018·International Journal of Molecular Sciences·Eunji KimJae Youl Cho

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA

Software Mentioned

GraphPad Prism
GraphPad

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here