Effect of Jagged-1 and Dll-1 on osteogenic differentiation by stem cells from human exfoliated deciduous teeth

Archives of Oral Biology
Waleerat SukarawanThanaphum Osathanon

Abstract

The aim of the present study was to determine the influence of Notch ligands, Jagged-1 and Dll-1, on osteogenic differentiation by stem cells from human exfoliated deciduous teeth. Notch ligands were immobilized on tissue culture surface using an indirect affinity immobilization technique. Cells from the remaining of dental pulp tissues from human deciduous teeth were isolated and characterized using flow cytometry and differentiation assay. Alkaline phosphatase (ALP) enzymatic activity, osteogenic marker gene expression, and mineralization were determined using ALP assay, real-time polymerase chain reaction, and alizarin red staining, respectively. The isolated cells exhibited CD44, CD90, and CD105 expression but lack of CD45 expression. Further, these cells were able to differentiate toward osteogenic lineage. The upregulation of HES-1 and HEY-1 was observed in those cells on Dll-1 and Jagged-1 coated surface. The significant increase of ALP activity and mineralization was noted in those cells seeded on Jagged-1 surface and these results were attenuated when cells were pretreated with gamma secretase inhibitor. The significant upregulation of ALP and collagen type I gene expression was also observed in those cells seeded on J...Continue Reading

References

Oct 3, 2001·The Journal of Experimental Medicine·A C JalecoL Parreira
Jan 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ken-Ichi TezukaNobumichi Hozumi
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Jul 29, 2005·European Journal of Oral Sciences·H LøvschallK Poulsen
Jun 8, 2006·Journal of Biomedical Materials Research. Part a·Benjamin L BecksteadCecilia M Giachelli
Aug 5, 2006·Journal of Cellular Biochemistry·Motohiro KomakiIsao Ishikawa
Feb 26, 2008·Journal of Dental Research·C ZhangC-Y Wang
May 27, 2008·Journal of Dental Research·S WongkhanteeP Pavasant
Nov 6, 2008·Journal of Biomedical Materials Research. Part a·Benjamin L BecksteadCecilia M Giachelli
Aug 22, 2009·Journal of Molecular and Cellular Cardiology·Vishal Nigam, Deepak Srivastava
Feb 16, 2010·Molecular and Cellular Biology·Saravana Kumar Ramasamy, Nibedita Lenka
Feb 19, 2010·Journal of Biomedical Materials Research. Part a·Boontharika ChuenjitkuntawornPrasit Pavasant
Apr 17, 2010·Journal of Dental Research·V T SakaiJ E Nör
Jun 17, 2011·Advances in Dental Research·T A MitsiadisJ Catón
Aug 6, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael I DishowitzKurt D Hankenson
Sep 29, 2011·Biochemical and Biophysical Research Communications·Yoko AoiOlga Safronova
Mar 7, 2012·Arthritis Research & Therapy·Chiyoko SekineHideo Yagita
Jun 9, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Elsa MachadoPedro de Sousa Gomes
Jul 17, 2012·Journal of Prosthodontic Research·Hiroshi EgusaKentaro Akiyama
Aug 1, 2012·Journal of Biomedical Materials Research. Part a·Thanaphum OsathanonPrasit Pavasant
Jun 19, 2013·Journal of Biomedical Materials Research. Part a·Michael I DishowitzKurt D Hankenson
Aug 22, 2013·Calcified Tissue International·Shan ChenYangjin Bae
Dec 20, 2014·Archives of Oral Biology·Nunthawan NowwaroteThanaphum Osathanon

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Citations

Feb 19, 2019·Oral Diseases·Jaijam SuwanwelaThanaphum Osathanon
Jul 6, 2019·Frontiers in Genetics·Tlili BarhoumiMajid Alfadhel
Oct 13, 2020·Tissue Engineering. Part B, Reviews·Kathleen Zohorsky, Kibret Mequanint
Mar 3, 2021·Archives of Oral Biology·Jeeranan ManokawinchokeHiroshi Egusa
Feb 7, 2018·Archives of Oral Biology·Nunthawan NowwaroteThanaphum Osathanon
Jun 1, 2021·Archives of Oral Biology·Chatvadee KornsuthisoponDamrong Damrongsri
May 18, 2021·Journal of Oral Biology and Craniofacial Research·Damrong DamrongsriThanaphum Osathanon
Jul 9, 2021·Journal of Cell Communication and Signaling·Sachin Thomas, Bithiah Grace Jaganathan

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