Effect of jasplakinolide on the growth, encystation, and actin cytoskeleton of Entamoeba histolytica and Entamoeba invadens

The Journal of Parasitology
Asao MakiokaT Takeuchi

Abstract

The effect of jasplakinolide. an actin-polymerizing and filament-stabilizing drug, on the growth, encystation, and actin cytoskeleton of Entamoeba histolytica and Entamoeba invadens was examined. Jasplakinolide inhibited the growth of E. histolytica strain HM-1:IMSS and E. invadens strain IP-1 in a concentration-dependent manner, the latter being more resistant to the drug. The inhibitory effect of jasplakinolide on the growth of E. histolytica trophozoites was reversed by removal of the drug after exposure to 1 microM for 1 day. Encystation of E. invadens as induced in vitro was also inhibited by jasplakinolide. Trophozoites exposed to jasplakinolide in encystation medium for 1 day did not encyst after removal of the drug, whereas those exposed to the drug in growth medium for 7 days did encyst without the drug. The process of cyst maturation was unaffected by jasplakinolide. Large round structures were formed in trophozoites of both amoebae grown with jasplakinolide; these were identified as F-actin aggregates by staining with fluorescent phalloidin. Accumulation in trophozoites of both amoebae of actin aggregates was observed after culture in jasplakinolide. Also, E. invadens cysts formed from trophozoites treated with jaspl...Continue Reading

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Citations

Sep 24, 2009·PloS One·Araceli Castillo-RomeroJose Manuel Hernandez
Aug 6, 2002·Current Opinion in Microbiology·William A Petri
Mar 25, 2015·Parasitology·Sonja Frölich, Michael Wallach
May 17, 2012·PLoS Neglected Tropical Diseases·Ibne Karim M AliWilliam A Petri

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Methods Mentioned

BETA
electrophoresis
electron microscopy

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