Effect of junctional adhesion molecule-2 expression on cell growth, invasion and migration in human colorectal cancer

International Journal of Oncology
Huishan ZhaoWen G Jiang

Abstract

The junctional adhesion molecule (JAMs) family belongs to the immunoglobulin subfamily involved in the formation of tight junctions (TJ) in both endothelial and epithelial cells. Aberrant expression of JAM-2 is associated with cancer progression but little work has been carried out in discovering how this affects changes in cell behaviour. The present study aimed to examine the expression of JAM-2 in human colon cancer specimens and cell lines and its role in the development of colon cancer. JAM-2 expression in human colon cancer specimens (normal, n=75; cancer, n=94) and cell lines was analysed using quantitative real-time PCR and conventional RT-PCR. Colon cancer cells were stably transfected with a mammalian expression vector to overexpress JAM-2-Flag. The effect on growth, adhesion and migration following overexpression of JAM-2 was then investigated using in vitro models. TJ function was assessed using a trans-epithelial resistance assay (TER, with an EVOM voltammeter). JAM-2 was lowly expressed in colon cancer cells such as RKO, HT115. JAM-2 overexpression in RKO cells (RKO-JAM-2) and HT115 cells (HT115-JAM-2) showed retarded adhesion (P<0.05). An in vivo tumour model showed that RKO-JAM-2 had significantly reduced growth...Continue Reading

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Citations

Jan 31, 2019·Frontiers in Physiology·Marianela González-GonzálezMarcela Julio-Pieper

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Methods Mentioned

BETA
surgical resections
PCR
Protein Assay
electrophoresis
FCS
zymography
reverse transcription-PCR

Software Mentioned

ImageJ
Minitab
Beacon Design

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