PMID: 9182252Feb 1, 1996Paper

Effect of K+- and kainate-mediated depolarization on survival and functional maturation of GABAergic and glutamatergic neurons in cultures of dissociated mouse cerebellum

Neurochemical Research
I DamgaardA Schousboe

Abstract

The effect of the depolarizing agents, an elevated potassium concentration (25 mM) or kainic acid (50 microM) on neuronal survival and differentiation was investigated in cultures of dissociated neurons from cerebella of 7-day-old mice. When maintained in the presence of an antimitotic agent such cultures consist primarily of glutamatergic and GABAergic neurons. Cell survival was monitored by measurement of DNA, and differentiation by determining uptake and depolarization coupled release of glutamate (D-aspartate as label) and GABA. The depolarizing agents were added separately or together either from the start of the culture period (7-8 days) or at day 5 in culture. The main findings are that K+ depolarization is important for differentiation of glutamatergic neurons but not for GABAergic neurons. This depolarizing signal is important during the early phase of development in culture. For glutamatergic neurons, kainate may replace K+ as a depolarizing signal whereas in case of the GABAergic neurons, kainate was toxic particularly during the late phase of development. It was further observed that the glutamatergic neurons when maintained in a medium with 5 mM K+ during the first 5 days in culture became sensitive to kainate toxi...Continue Reading

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Citations

Jul 6, 2002·European Journal of Pharmacology·Liang Peng, Leif Hertz
Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·H S Mogensen, O S Jorgensen
Jun 9, 2000·Annual Review of Neuroscience·J L Goldberg, B A Barres
Nov 24, 2004·Journal of Neuroscience Research·Helle WaagepetersenUrsula Sonnewald
Apr 29, 2004·Journal of Neuroscience Research·Mónica L Fiszman, Arne Schousboe
Dec 24, 1997·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M BardoulN König

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