Effect of kisspeptin antagonist on goat in vitro Leydig cell steroidogenesis

Theriogenology
Haney SamirGen Watanabe

Abstract

Kisspeptin is the protein product of Kiss1 gene. In contrast to its fundamental role as GnRH Secretagogue and puberty initiation, its functions in extrahypothalmic tissues such as the testes remain unknown. The objective of this study was to investigate the potential local role of kisspeptin in the Leydig cell culture in goats. Five male Shiba goats were castrated to isolate Leydig cells from the testes. The isolated Leydig cells were then purified and cultured with different concentration of kisspeptin antagonist (0, 5, and 10 nmol; 0KPA, 5KPA, and 10KPA; respectively). After 12 h, 20 IU of hCG was added to some wells (0KPA + hCG, 5KPA + hCG, and 10KPA + hCG; respectively); other wells did not receive hCG (0KPA + Con, 5KPA + Con, and 10KPA + Con; respectively). Media of Leydig cell culture was harvested at 2, 6, 12, and 24 h after addition of hCG for testosterone (T) and estradiol (E2) assays. The contents of some non KPA treated wells (0KPA + Con and 0KPA + hCG wells) were collected and stored at -80 OC for further identification the expression of mRNA encoding Kiss1 and its receptor (GPR54) using real time PCR. Results revealed high expressions of Kiss1/GPR54 mRNAs in the hCG treated (0KPA + hCG) wells compared to non-hCG tr...Continue Reading

Citations

Aug 11, 2019·Reproductive Biology and Endocrinology : RB&E·Yubin CaoHaibin Kuang
Jan 8, 2020·Journal of Clinical Medicine·Víctor BlascoManuel Fernández-Sánchez
Dec 16, 2019·Journal of Endocrinological Investigation·A SzeligaB Meczekalski
Sep 21, 2020·General and Comparative Endocrinology·Rosaria MeccarielloRiccardo Pierantoni

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