DOI: 10.1101/499889Dec 19, 2018Paper

Effect of KNL1 on the proliferation and apoptosis of colorectal cancer cells

BioRxiv : the Preprint Server for Biology
Tianliang BaiBinghui Li

Abstract

Background: To identify the expression of KNL1 in colorectal tumor tissues and to clarify the function of this gene on the proliferation capability of colorectal cancer cells. Methods: Thirty-six pairs of colorectal tumor and normal tissue were collected and subjected to quantitative PCR analysis. KNL1 was under-expressed using lentiviral transfection, and the function of KNL1 was evaluated by proliferation assay, colony formation and apoptosis assay. Results: KNL1 was highly expressed in colorectal tumor tissues. KNL1 downregulation inhibited colorectal cancer cell proliferation and promoted apoptosis. Conclusions: KNL1 plays an effective role in decreasing the apoptosis and promoting the proliferation of colorectal cancer cells.

Related Concepts

Colorectal Neoplasms
Down-Regulation
Genes
Apoptosis
Tumor Cells, Malignant
Cell Proliferation
Protein Expression
CASC5 protein, human
Colorectal Cancer
Real-Time Polymerase Chain Reaction

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