Effect of L-methionine and S-adenosylmethionine on growth of an adenine mutant of Saccharomyces cerevisiae.

Journal of Bacteriology
I YallR C Knudsen

Abstract

A pink, adenine-requiring yeast utilized adenine, hypoxanthine, or S-adenosylmethionine (SAM), in quantities up to 3 mumoles per 100 ml of medium, as equivalent sources of purine for cell growth, but not methylthioadenosine or S-adenosylhomocysteine. Utilization of SAM for growth was inhibited by the presence of l-methionine in quantities greater than 0.6 mumole per 100 ml of medium. However, 6 mumoles of l-methionine had no effect on growth when adenine or hypoxanthine was the source of purine. These sources also reversed the inhibitory effects of 6 mumoles of the amino acid on the utilization of SAM. The presence of 400 mumoles of the amino acid resulted in some inhibition of growth when the organisms were grown with adenine, hypoxanthine, or adenine plus SAM but had no effect on the total uptake of adenine-8-(14)C. Studies on the uptake of radioactivity from a mixture of SAM-adenine-8-(14)C and (3)H-labeled SAM-methyl indicated that these components were taken into the cells at different rates which were altered by the presence of l-methionine. The fixation of (35)S from (35)S-labeled adenosylmethionine into the cells was inhibited by the presence of the amino acid. The cells synthesized and accumulated SAM in the presence o...Continue Reading

References

Oct 1, 1966·Journal of Bacteriology·J W Daniel, K Babcock
Mar 1, 1962·Archives of Biochemistry and Biophysics·J A DUERRE, F SCHLENK
Jan 1, 1963·Archives of Biochemistry and Biophysics·S K SHAPIROM HERTENSTEIN
Jul 20, 1964·Archives of Biochemistry and Biophysics·F SCHLENK, D J EHNINGER
Jun 1, 1962·Archives of Biochemistry and Biophysics·C J PIGGL W PARKS

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