Effect of L-NAME on pressure-flow relationships in isolated rabbit lungs: role of red blood cells

The American Journal of Physiology
R S SpragueN A Weintraub

Abstract

Nitric oxide (NO) is produced by and relaxes pulmonary arteries and veins; however, a role for NO as a participant in the control of pulmonary vascular resistance (PVR) remains to be defined. Here we investigated the hypothesis that for NO to serve as a determinant of PVR in the rabbit requires the presence of blood. In isolated blood-perfused rabbit lungs, NG-nitro-L-arginine methyl ester (L-NAME, 100 microM) increased PVR and the slope of the pressure-flow relationship. These effects of L-NAME were prevented by pretreatment with L-arginine. In contrast, in lungs perfused with a physiological salt solution, L-NAME had no effect on PVR or the pressure-flow relationship. The addition of washed red blood cells (RBCs) to physiological salt solution, but not the addition of plasma and platelets, restored the response to L-NAME. This effect of RBCs was not reproduced by increasing perfusate viscosity with dextran. These results suggest that, in the rabbit lung, NO is a determinant of PVR in the presence of blood. Moreover, that aspect of blood that permits the generation of NO appears to be related to the RBC and not to perfusate viscosity.

Citations

Jan 28, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·John B GordonJane A Madden
Dec 13, 2005·American Journal of Physiology. Cell Physiology·Barbara A KlarlFlorian Lang
Dec 14, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Griffith LiangRandy S Sprague
Dec 14, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Meera SridharanRandy S Sprague
Sep 19, 2020·Experimental Lung Research·Maurizio TurzoCornelius J Busch
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J T BergE R Swenson
Apr 12, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Randy S SpragueAndrew J Lonigro
Mar 22, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Fabian SpöhrJörg Weimann
Sep 8, 2001·American Journal of Physiology. Cell Physiology·R S SpragueA J Lonigro
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Girija G Konduri, Janine Mattei

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