Effect of Lactobacillus plantarum and Pseudomonas aeruginosa culture supernatants on polymorphonuclear damage and inflammatory response

International Immunopharmacology
A N RamosJuan C Valdez

Abstract

In a previous study we determined that by-products of Lactobacillus plantarum inhibited pathogenicity of Pseudomonas aeruginosa and is effective in the treatment of infected wounds. This study assesses the cytotoxic activity of acetic acid (AA), supernatants of L. plantarum and P. aeruginosa, with and without signal acyl-homoserine-lactones (AHL), and mixtures of both bacterial supernatants on human neutrophils. Cytotoxicity was determined through viability using trypan blue, apoptosis by Annexin V, necrosis by propidium iodide and intracellular pH by SNARF-1. We found that supernatants of L. plantarum caused less cytotoxicity than AA at the same extracellular pH (p<0.05). P. aeruginosa induced a remarkable drop in intracellular pH, which was independent of extracellular pH. This intracellular acidity was correlated with a significant decrease in viability and was higher than supernatants of AHL producing P. aeruginosa (p<0.05). When supernatants were mixed, the quantity of AHL diminished (p<0.001) and the cytotoxic effect induced by P.aeruginosa was ameliorated by L. plantarum supernatant (p<0.001 vs p<0.01). These results are in agreement with the inflammatory in vivo assays determined by intradermal inoculations in Balb/c mi...Continue Reading

References

Jun 1, 1993·Journal of the Royal Army Medical Corps·J M SlossS M Milner
Dec 29, 1998·Emerging Infectious Diseases·C Van Delden, B H Iglewski
May 21, 1999·Science·J W CostertonE P Greenberg
Jan 4, 2001·Microbes and Infection·K P RumbaughA N Hamood
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Jan 29, 2008·Journal of the American Academy of Dermatology·Margaret A FonderAdam J Mamelak
Mar 18, 2009·International Wound Journal·Maria C PeralJuan C Valdez
Jun 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C PeralJ C Valdez

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Citations

May 31, 2012·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Alberto N RamosJuan C Valdez
Feb 18, 2015·Pharmaceutical Development and Technology·Carla Agostina CabreraMaria Eugenia Sesto Cabral
Jul 9, 2014·Pharmaceutical Development and Technology·Maria Eugenia Sesto CabralJuan Carlos Valdez
Jul 6, 2014·Clinical and Vaccine Immunology : CVI·M T NieminenR Rautemaa
Jul 28, 2019·Journal of Translational Medicine·M BesserE K Stuermer

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