Effect of lentinan and mannan on phagocytosis of fluorescent latex microbeads by mouse peritoneal macrophages: a flow cytometric study.

International Journal of Immunopharmacology
G AbelJ Fachet

Abstract

Lentinan, an immunopotentiating beta-1,3-glucan polysaccharide stimulated the in vitro phagocytosis of BSA-coated, C3b- or monoclonal immunoglobulin (IgG2b)-coated fluorescent microspheres by resident or thioglycollate-elicited mouse macrophages in a dose-dependent manner. Analysis of flow cytometric data has shown that microbead phagocytosis of resident macrophages, which exhibit a lower basic phagocytic activity than the thioglycollate elicited ones, has been augmented by up to 900% due to lentinan. The percent ratio of phagocytes among peritoneal exudate cells, however, remained unchanged after short-term lentinan stimulation. Preincubation of the cells with lentinan resulted in increased ingestion of the microbeads. Activation of phagocytosis by lentinan is therefore due in part to the direct stimulation of the cells, however, lentinan also serves as supplementary opsonin for C3b-coated beads. Mannan inhibited the ingestion of C3b-coated microspheres by 75%, which was abolished in part when lentinan was also added to the cells. Mannan did not influence the phagocytosis of BSA-coated or IgG-coated beads. Our data, based solely on in vitro studies, suggest a beta-glucan receptor mediated activation of phagocytes by lentinan. ...Continue Reading

References

Jun 11, 1975·The Journal of Experimental Medicine·C BiancoS C Silverstein
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J K Czop, K F Austen
Mar 1, 1987·Scandinavian Journal of Immunology·P ArturssonJ L Ericsson
Jan 15, 1986·The Biochemical Journal·S HiraniH J Müller-Eberhard
Oct 1, 1972·The Journal of Cell Biology·R M Steinman, Z A Cohn
Jan 1, 1983·The Journal of Cell Biology·S S SungS C Silverstein
Dec 15, 1980·International Journal of Cancer. Journal International Du Cancer·J ZákányJ Fachet

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Citations

Jan 1, 1993·Cancer Immunology, Immunotherapy : CII·A LadányiK Lapis
Sep 14, 1994·Journal of Immunological Methods·N Plasman, B Vray
May 12, 2004·International Immunopharmacology·Vaclav Vetvicka, Jean-Claude Yvin
Jun 1, 1996·International Journal of Immunopharmacology·C KerékgyártóJ Fachet
Feb 1, 1997·Vaccine·J C Cox, A R Coulter
Aug 30, 2003·International Immunopharmacology·Nadya MarkovaTatyana Radoucheva
Dec 5, 2008·Journal of Medicinal Food·Vaclav VetvickaJana Vetvickova
Jun 18, 2010·Journal of Medicinal Food·Vaclav VetvickaJean-Claude Yvin
Dec 29, 2010·Journal of Medicinal Food·Sujata Saraswat-OhriVincent Ferrières
Aug 1, 1991·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·G AbelJ Fachet
May 25, 2013·Journal of Clinical Biochemistry and Nutrition·Masashi Mizuno, Yosuke Nishitani
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
Jul 3, 2007·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Vaclav VetvickaJean-Claude Yvin
Sep 17, 2002·The American Journal of Chinese Medicine·Tsutomu NakadaToshihiko Hanawa
Mar 24, 2006·Immunology Letters·M Y K LeungK P Fung
Aug 11, 2007·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Vaclav VetvickaJana Vetvickova
Apr 22, 2011·Expert Review of Vaccines·Nikolai Petrovsky, Peter D Cooper
Sep 19, 2006·International Journal of Biological Macromolecules·Vaclav VetvickaJean-Claude Yvin

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