Effect of levan's branching structure on antitumor activity

International Journal of Biological Macromolecules
Eun Ju YoonHyeon Gyu Lee

Abstract

Levan produced from Microbacterium laevaniformans KCTC 9732 (M-levan) was isolated and treated with an inulinase to modify its branching structure. The chemical structures of levans were characterized, and the modified levans were applied on animal tumor cells to evaluate their antitumor activity. The GC-MS analysis indicated that beta-(2,1)-linked branches of M-levan were specifically hydrolyzed. As the ratio of applied inulinase to levan increased, the branching degree decreased proportionally. Sequential degrees of branching were obtained from 12.3 to 4.2%. Strong levan-induced inhibition of cell growth was detected on SNU-1 and HepG2 tumor cell lines. As the branching degree of M-levan reduced, antitumor activity on SNU-1 linearly decreased (r2=0.96). In HepG2, the antitumor activity rapidly dropped when the branching reached up to 9.3%, then slightly increased as the branching degree of M-levan further decreased. These results suggested that the branch structure would play a crucial role in levan's antitumor activity.

References

Dec 29, 1999·FEMS Microbiology Letters·S P MarxW Hartmeier

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Citations

Dec 17, 2010·Applied Microbiology and Biotechnology·Faruk KüçükaşikEbru Toksoy Oner
Mar 2, 2013·Indian Journal of Microbiology·Seema PatelArun Goyal
Nov 20, 2008·Applied Microbiology and Biotechnology·Chung-Jen ChiangYun-Peng Chao
Nov 26, 2015·PloS One·Luz Méndez-LorenzoAgustín López Munguía
Mar 24, 2006·Immunology Letters·M Y K LeungK P Fung
Sep 6, 2013·FEMS Microbiology Letters·Alan J MarshPaul D Cotter
Dec 17, 2018·Carbohydrate Polymers·Artur Szwengiel, Ghomaka Lydia Nkongha
May 6, 2021·Environmental Science and Pollution Research International·Anissa HaddarSemia Ellouz-Chaabouni
Feb 1, 2019·International Journal of Biological Macromolecules·Artur Szwengiel, Maciej Wiesner

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