Effect of LGI1 antibody-positive IgG on hippocampal neuron survival: a preliminary study

Neuroreport
Neşe Ayşit-AltuncuErdem Tüzün

Abstract

Anti-leucine-rich glioma inactivated 1 (anti-LGI1) encephalitis is one of the most frequently encountered forms of autoimmune encephalitis. Many patients with anti-LGI1 encephalitis develop permanent hippocampal neuron loss and chronic neuropsychiatric symptoms, suggesting that LGI antibodies (Ab) might have a neurotoxic action. To investigate this hypothesis, purified serum IgG of three patients with anti-LGI1 encephalitis and six healthy controls were incubated with cultured primary hippocampal neurons obtained from newborn mice. Nontreated cells were used as controls. The viability of IgG-treated neurons was evaluated by propidium iodide staining. Apoptotic mechanisms were assessed by JC-1 assay and mRNA expression level measurement of apoptosis-related genes using real-time PCR. The effect of IgG treatment on calcium influx was analyzed by fluo-4 calcium imaging. LGI1-Ab+ IgG increased the number of propidium iodide positive neurons, reduced mitochondrial membrane potentials, upregulated caspase-3 and Bax mRNA expression levels and downregulated Bcl-2 mRNA expression levels of neurons. LGI1-Ab+ IgG-treated neurons showed lower calcium staining than healthy controls IgG-treated and non-IgG-treated neurons. Our results indica...Continue Reading

References

Oct 1, 1993·The Journal of Pathology·G CattorettiE Feudale
Mar 7, 2006·Journal of Cellular Physiology·Nadia GabelliniG Palù
Mar 8, 2006·The European Journal of Neuroscience·Sara BondeOlle Lindvall
Mar 19, 2011·Biochemical and Biophysical Research Communications·Jean-Yves Tano, Guillermo Vazquez
Apr 28, 2012·Brain : a Journal of Neurology·Christian G BienJan Bauer
Nov 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Toshika OhkawaMasaki Fukata
Feb 26, 2016·Lancet Neurology·Francesc GrausJosep Dalmau
Apr 12, 2016·Neurology. Genetics·Mikko MuonaAnna-Elina Lehesjoki
Oct 21, 2016·Neuroscience Research·Yuko FukataMasaki Fukata
Apr 4, 2017·Brain : a Journal of Neurology·Thomas D MillerClive R Rosenthal
Mar 1, 2018·The New England Journal of Medicine·Josep Dalmau, Francesc Graus

❮ Previous
Next ❯

Citations

Jun 27, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Khalil S Husari, Divyanshu Dubey
Oct 8, 2020·Journal of Neural Transmission·Niels Hansen, Charles Timäus
Oct 27, 2020·Epilepsia·Eleonora LugaràMatthew C Walker

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

ImageJ
Zeiss Axiovision
primer3

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.