Effect of life stage and pesticide exposure on the gut microbiota of Aedes albopictus and Culex pipiens L.

Scientific Reports
Elijah O JumaEphantus J Muturi

Abstract

Pesticides commonly contaminate the aquatic environments inhabited by mosquito juveniles. However, their role in shaping the mosquito microbiota is not well understood. We hypothesized that environmentally relevant concentrations of atrazine, permethrin and malathion will mediate a shift in the mosquito gut bacterial community structure due to their toxic effect on the aquatic bacterial communities, and reduce mosquito gut bacterial diversity by enriching pesticide-degrading bacterial communities over susceptible taxa. Illumina MiSeq sequencing of the V3-V4 hypervariable regions of the 16 S rRNA gene was used to characterize the microbial communities of larval and adult stages of the two mosquito species and the water samples from microcosms treated with each of the pesticides, separately. Bacterial community composition differed by sample type (larval stage vs. adult stage) and water sampling date (day 3 vs. day 7), but not by pesticide treatment. In larval stages, bacterial OTU richness was highest in samples exposed to malathion, intermediate in permethrin, and lowest in controls. Bacterial richness was significantly higher in larval stages compared to adult stages for all treatments. This study provides a primer for future ...Continue Reading

References

Apr 1, 1997·The American Journal of Tropical Medicine and Hygiene·Y B MebrahtuM Taylor
Jul 1, 1997·Emerging Infectious Diseases·C G Moore, C J Mitchell
Aug 1, 2000·Journal of Medical Entomology·R S NasciE S Brannon
Aug 19, 2000·Archives of Environmental Contamination and Toxicology·C D MilamJ D Wilhide
Aug 4, 2001·Environment International·M GraymoreG Allinson
Sep 26, 2001·Applied and Environmental Microbiology·J B HughesB J Bohannan
Feb 11, 2004·Environmental Toxicology and Chemistry·Sangjin LeeDavid E Crowley
Apr 21, 2005·Environmental Toxicology and Chemistry·Erin L AmwegNicole M Ureda
Dec 14, 2005·Applied and Environmental Microbiology·M L de SouzaL P Wackett
Mar 2, 2006·Journal of the American Mosquito Control Association·Bryan T JacksonBelinda Hawkins
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Aug 28, 2007·American Journal of Epidemiology·Matthew R BonnerMichael C R Alavanja
Feb 4, 2010·Pest Management Science·L Jason KrutzSteven J Thomson
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Sep 8, 2010·Environmental Toxicology and Chemistry·Yuping DingMichael J Lydy
Sep 11, 2012·Applied and Environmental Microbiology·Zachery R StaleyValerie J Harwood
Nov 6, 2012·Insect Biochemistry and Molecular Biology·Theresia Estomih NkyaJean-Philippe David
Nov 28, 2012·Current Opinion in Biotechnology·Terry C HazenStephen M Techtmann
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
May 22, 2013·Parasites & Vectors·Guillaume MinardClaire Valiente Moro
Mar 7, 2014·Ground Water·Patricia L ToccalinoMichael G Rupert
Apr 29, 2014·Molecular Ecology·Kerri L CoonMichael R Strand
Oct 7, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Geoffrey GimonneauIsabelle Morlais
Nov 7, 2014·Critical Reviews in Toxicology·Glen J Van Der KraakKeith R Solomon
Dec 3, 2014·Journal of Insect Physiology·Andrea VogelAnthony C Fiumera
Jan 22, 2015·Nucleic Acids Research·Matthew E RitchieGordon K Smyth
Nov 14, 2015·Critical Reviews in Toxicology·Zachery R StaleyJason R Rohr
Apr 27, 2016·Journal of Medical Entomology·Dana L VanlandinghamYan-Jang S Huang
May 14, 2016·Nature Microbiology·Xiaojing PangGong Cheng

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Citations

Jun 3, 2021·International Journal of Environmental Research and Public Health·Federica GiambòConcettina Fenga
Sep 28, 2021·Frontiers in Microbiology·Arley Calle-TobónBerlin Londoño-Rentería

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Methods Mentioned

BETA
Illumina sequencing
PCR

Software Mentioned

RStudio environment
RStudio
R package “ npmv
CLC genomics workbench
ORD
QIIME
PC
R package “ limma ”
phyloseq
R

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