Effect of ligand conformation on melanoma cell alpha3beta1 integrin-mediated signal transduction events: implications for a collagen structural modulation mechanism of tumor cell invasion

Biochemistry
J L LauerG B Fields

Abstract

The importance of three-dimensional interactions between receptors with their respective ligands has been extensively explored during the binding process, but considerably less so for postbinding events such as induction of signaling pathways. Tumor cell receptor association with basement membrane proteins is believed to facilitate the metastatic process. Melanoma and ovarian carcinoma cells have been shown to utilize the alpha3beta1 integrin to bind to models of the alpha1(IV)531-543 sequence from basement membrane (type IV) collagen [Miles, A. J., et al. (1994) J. Biol. Chem. 269, 30939-30945; Miles, A. J., et al. (1995) J. Biol. Chem. 270, 29047-29050]. In the present study, the effects of ligand three-dimensional structure on possible signal transduction pathways induced by alpha3beta1 integrin binding have been evaluated. Human melanoma cell binding to type IV collagen resulted in Tyr phosphorylation of p125(FAK), consistent with prior studies correlating beta1 integrin subunit binding to collagen and p125(FAK) Tyr phosphorylation. Cross-linking of an anti-alpha3 integrin subunit monoclonal antibody also induced p125(FAK) Tyr phosphorylation. Incubation of melanoma cells with single-stranded or triple-helical peptide model...Continue Reading

References

Dec 1, 1993·Melanoma Research·I G YoshinagaH R Byers
Mar 1, 1996·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·M AchisonR W Farndale

❮ Previous
Next ❯

Citations

May 9, 2006·Cancer Metastasis Reviews·H G Munshi, M S Stack
Mar 24, 2004·Critical Reviews in Oncology/hematology·Sylvie PascoJean Claude Monboisse
Jan 1, 1997·Trends in Biochemical Sciences·T Hunter, G D Plowman
Mar 6, 2010·Organic & Biomolecular Chemistry·Gregg B Fields
May 23, 2003·Journal of Biomedical Materials Research. Part a·Catherine D Reyes, Andrés J García
Jan 26, 2008·Microscopy Research and Technique·Jamshid KhoshnoodiBilly G Hudson
Apr 24, 2012·Annals of Neurology·Yi-Chinn WengDouglas B Gould
Mar 23, 2005·Biochimie·F X MaquartJ C Monboisse
Jun 7, 2005·Cancer Detection and Prevention·Sylvie PascoJean-Claude Monboisse
Feb 4, 2003·Immunology·Farah D LubinDennis W McGee
Jun 25, 2014·The Journal of Biological Chemistry·Maciej J StawikowskiGregg B Fields
May 2, 2000·The Journal of Biological Chemistry·J L Lauer-FieldsG B Fields
Mar 19, 2003·The Journal of Biological Chemistry·Janelle L Lauer-FieldsGregg B Fields
Nov 22, 2001·Bioconjugate Chemistry·W B EdwardsM J Welch
May 14, 2003·Biomacromolecules·Navdeep B MalkarGregg B Fields

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.