PMID: 8948437Jun 15, 1995Paper

Effect of lipopolysaccharide on mitogen-activated protein kinases and cytosolic phospholipase A2

The Biochemical Journal
S I FoudaR I Sha'afi

Abstract

The addition of platelet-activating factor (PAF) to human neutrophils increases phosphorylation on tyrosine residues and stimulates the activity of p42erk2 mitogen-activated protein kinase (MAP kinase). This action is rapid and transient. In contrast, p42erk2, p44erk1 and the p40hera MAP kinase isoforms are all not tyrosine phosphorylated or activated in human neutrophils stimulated with low concentrations of lipopolysaccharide (LPS) in combination with serum. In spite of this, the PAF-induced tyrosine phosphorylation and activation of the p42erk2 MAP kinase are greatly potentiated in cells pretreated with LPS. More interestingly, although low concentrations of LPS do not affect MAP kinase isoforms in these cells, they cause the phosphorylation of cytosolic phospholipase A2 (cPLA2), as evidenced by a decrease in the electrophoretic mobility of the enzyme. In addition, this stimulus-induced upward shift in the mobility of the enzyme is not inhibited by the tyrosine kinase inhibitor, genistein. Furthermore, LPS increases the release of arachidonic acid in control and PAF-stimulated human neutrophils. These observations clearly show that cPLA2 can be phosphorylated and activated by kinases other than the currently known MAP kinase...Continue Reading

Citations

Oct 12, 1999·The American Journal of Physiology·H ParfenovaC W Leffler
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J A NickG S Worthen
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jerry A NickG Scott Worthen
Aug 7, 1999·Clinical Reviews in Allergy & Immunology·M C Seeds, D A Bass
Jul 4, 1997·The Journal of Biological Chemistry·C C Leslie
Nov 7, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M E SuretteP Borgeat
Feb 22, 2003·The Journal of Biological Chemistry·Kenneth C Malcolm, G Scott Worthen
Sep 25, 2003·Physiological Reviews·Bjarne Osterud, Eirik Bjorklid
Oct 28, 1998·Physiological Reviews·E J Pettit, F S Fay
Oct 16, 2002·Biochimica Et Biophysica Acta·Paul R S BakerRobert L Wykle
Nov 5, 1999·Clinical and Diagnostic Laboratory Immunology·I DanielsA P Haynes
May 14, 2003·Cellular Signalling·Sajal Chakraborti

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