Effect of lipopolysaccharide on mouse mast cell induction by a splenic cell culture system.

Infection and Immunity
Z Q HuT Shimamura

Abstract

We have previously reported a method of mast cell induction by long-term culture of mouse spleen cells without using exogenous mast cell growth factor (Z.-Q. Hu, T. Yoshida, and T. Shimamura, J. Immunol. Methods 149:173, 1992). Supernatants recovered from the long-term cultures contain endogenous interleukin 3 and soluble stem cell factor. These were assessed by the capacity of the recovered supernatants to foster the growth of a mast cell growth factor-dependent cell line and by neutralizing antibodies. Besides the soluble factors, cell-to-cell contacts mediated by membrane stem cell factor on splenic stromal cells and c-Kit receptors on mast cells also affect mast cell induction. Different lots of fetal calf serum (FCS) were examined to determine a possible trigger for cytokine production. FCS can be divided into mast cell-inducible and noninducible sera by this process. However, not all FCS lots contain mast cell growth factor. The mast cell-inducible lots contain lipopolysaccharide (LPS) confirmed by a Limulus assay. Polymyxin B can neutralize the mast cell induction activity. Non-mast cell-inducible FCS can be converted to inducible FCS by adding exogenous LPS. The results indicate that LPS as a trigger of cytokine product...Continue Reading

References

Oct 5, 1990·Cell·D E WilliamsH S Boswell
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Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E RazinR A Good

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Citations

Feb 24, 2006·International Archives of Allergy and Immunology·K Matsui, A Nishikawa
Mar 8, 2006·Immunology and Cell Biology·Zhi-Qing HuTadakatsu Shimamura
Apr 7, 2006·European Journal of Immunology·Zhi-Qing HuStephen J Galli

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