Effect of lipopolysaccharides on vascular endothelial growth factor expression in mouse pulp cells and macrophages

European Journal of Oral Sciences
Tatiana BoteroJ E Nör

Abstract

Vascular endothelial growth factor (VEGF), a potent pro-angiogenic factor, might regulate the neovascularization observed in the pulp of teeth with deep caries. The purpose of this in vitro study was to evaluate the effect of bacterial lipopolysaccharides (LPS) on VEGF expression in dental pulp cells. Mouse odontoblast-like cells (MDPC-23) or undifferentiated pulp cells (OD-21) were exposed to 0-20 microg ml-1Escherichia coli LPS or 0-80 microg ml-1Prevotella intermedia LPS. As controls, mouse macrophages or gingival fibroblasts were exposed to LPS, since these cells are known to secrete VEGF. The VEGF expression was evaluated by reverse transcriptase polymerase chain reaction or enzyme-linked immunosorbent assay. The baseline expression levels of VEGF protein were higher in MDPC-23 and OD-21 than in fibroblasts or macrophages. Vascular endothelial growth factor protein expression was upregulated in MDPC-23 and macrophages exposed to E. coli LPS, but not in OD-21 cells or fibroblasts. Higher concentrations of P. intermedia LPS were required to induce VEGF expression in MDPC-23 cells. Treatment with LPS did not affect VEGF expression at the mRNA level in any of the cells evaluated. These results demonstrate that bacterial LPS up...Continue Reading

References

Oct 1, 1985·Journal of Dental Research·E Hoshino
Jan 1, 1973·Caries Research·W J Loesche, S A Syed
Jan 1, 1980·Caries Research·G RøllaK W Knox
Mar 1, 1995·Journal of Endodontics·A NakaneH Nakamura
Oct 1, 1994·Oral Surgery, Oral Medicine, and Oral Pathology·G Sundqvist
Nov 1, 1994·Oral Surgery, Oral Medicine, and Oral Pathology·S Seltzer, P A Farber
May 1, 1993·In Vitro Cellular & Developmental Biology. Animal·R J BingA Conforto
Apr 1, 1993·Immunobiology·E T RietschelS Kusumoto
Feb 1, 1993·Oral Microbiology and Immunology·W L MasseyW R Hume
Feb 2, 1996·The Journal of Biological Chemistry·A P LevyM A Goldberg
Jan 1, 1995·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P J Polverini
Jan 1, 1996·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D H Pashley
Dec 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·N Ferrara
Apr 16, 1998·European Journal of Oral Sciences·C T HanksW T Butler
Dec 23, 1998·Connective Tissue Research·C T HanksW T Butler
Mar 6, 1999·Current Opinion in Microbiology·R P Darveau
Sep 23, 2000·Archives of Oral Biology·D J Roberts-Clark, A J Smith
Mar 21, 2001·Cellular Signalling·M Guha, N Mackman
May 19, 2001·International Immunopharmacology·O Takeuchi, S Akira
Aug 30, 2001·Nature Immunology·T HorngR Medzhitov
Jan 25, 2002·Journal of Endodontics·Luciano ArteseAdriano Piattelli
Apr 12, 2002·The Lancet Infectious Diseases·Isaac Ginsburg
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R M Love, H F Jenkinson

❮ Previous
Next ❯

Citations

Jun 15, 2007·Critical Care Medicine·Po-Nien TsaoSu-Ming Hsu
Feb 22, 2012·Journal of Endodontics·Yusuke YamanakaTakashi Okiji
Nov 5, 2008·Archives of Oral Biology·B A SchevenA J Smith
Nov 27, 2007·Journal of Endodontics·Leticia Grando MattuellaAnna Christina Medeiros Fossati
Apr 18, 2007·Journal of Endodontics·Leticia Grando MattuellaAnna Christina Medeiros Fossati
Nov 1, 2011·International Dental Journal·Vinicius RosaJacques E Nör
Feb 8, 2014·Dental Traumatology : Official Publication of International Association for Dental Traumatology·Kathleen G NeivaRoberta Pileggi
Sep 27, 2005·Dental Materials : Official Publication of the Academy of Dental Materials·Maria G MantelliniJacques E Nör
Oct 15, 2014·International Endodontic Journal·M R ShinE-C Kim
Oct 1, 2016·Stem Cells International·Jessica RatajczakPetra Hilkens
Sep 22, 2009·Journal of Dental Research·R I SodenJ E Nör
Dec 5, 2012·Brazilian Dental Journal·Mayara Sgarbi SemeghiniKarina Fittipaldi Bombonato-Prado
Mar 12, 2011·Journal of Dental Research·A DiogenesK M Hargreaves
Jan 30, 2010·Journal of Dental Research·T M BoteroJ E Nör
Jul 1, 2015·Molecular and Cellular Biology·Hannah M HeairMohammad Q Hassan
May 14, 2020·Molecular Genetics & Genomic Medicine·Reza JafariReza Aflatoonian
Mar 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Claudia MedaEnrico Giraudo
Jan 1, 2010·The Journal of Clinical Pediatric Dentistry·Naveen ManujaSeema Chaudhary

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.