Effect of Liposome Treatment on Hemorheology and Metabolic Profile of Human Red Blood Cells During Hypothermic Storage

Biopreservation and Biobanking
Luciana da Silveira CavalcanteJelena L Holovati

Abstract

Ex vivo cold storage of red blood cells (RBCs) for transfusion has long been associated with hypothermic storage lesions. It has been proposed that liposomes can be used to mitigate hemorheological elements of RBC membrane storage lesions. This study aimed to determine the appropriate liposome treatment time and assess the effects of liposome treatment on RBC's hemorheological and metabolic profiles. Unilamellar liposomes were synthesized to contain a bilayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):cholesterol (7:3 mol%). Packed human RBCs (n = 4) were divided into untreated control (HEPES-NaCl solution) and liposome-treated samples (2 mM DOPC liposomes) and treated at days 2, 21, and 42 of hypothermic storage. RBC quality assessment included percent hemolysis, deformability, aggregation, hematological indices, microvesiculation, supernatant potassium, adenosine triphosphate (ATP), and 2,3-diphosphoglycerate (2,3-DPG). Among the parameters affected by liposome treatment time were deformability, aggregation amplitude (Amp), mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and microparticle mean fluorescence intensity. After 6 weeks of storage, aggregation index (AI) and Amp were significantly in...Continue Reading

References

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Apr 9, 2016·Journal of Liposome Research·Luciana da Silveira CavalcanteJelena L Holovati

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Citations

Jun 12, 2020·Biopreservation and Biobanking·Xiaozhang ZhangGang Zhao
Feb 12, 2021·International Journal of Molecular Sciences·Sylwia Cyboran-MikołajczykMagdalena Wawrzyńska

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

CellQuest
SPSS

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