Effect of local administration of platelet-rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model

Microsurgery
Akira IkumiMasashi Yamazaki

Abstract

This study aimed to evaluate the nerve regenerative effect and behavior of Schwann cells (SCs) on local administration of autologous platelet-rich plasma (PRP). Twenty-eight Japanese white rabbits were used. A 15-mm sciatic nerve defect was created on the left limb. The resected nerve was used as a reverse autologous nerve. The rabbits were randomly divided into two groups. In group A (n = 10), only nerve grafting was performed. In group B (n = 18), nerve grafting was performed with local PRP administration. Right limbs were used as control (group C, n = 28). The rabbits in each group were equally divided into two subgroups based on the evaluation period of 4 and 12 weeks after grafting. Electrophysiological evaluation, muscle wet-weight, histological evaluation, and multiple immunofluorescence staining were performed to investigate the regenerative effect of PRP. The mean regenerative axon diameter of the graft portion in group B (2.02 ± 0.22-μm) was significantly larger than that in group A (1.89 ± 0.16-μm) at 4 weeks. The regenerative axon number at the distal portion showed a greater increase in group B (9017 ± 2224/mm2 ) than in group A (4955 ± 3117/mm2 ) at 12 weeks. Electrophysiological evaluation and muscle wet-weight r...Continue Reading

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Citations

Nov 22, 2018·International Wound Journal·Oliver J SmithAsh Mosahebi
May 10, 2020·Tissue Engineering. Part B, Reviews·Jie FangYu Wang
Apr 28, 2020·Laryngoscope Investigative Otolaryngology·Carol H YanZara M Patel
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Jun 22, 2021·Plastic and Reconstructive Surgery·Akira IkumiPeter C Amadio
Jul 22, 2021·European Journal of Pain : EJP·Yohann BohrenEric Salvat
Sep 30, 2021·Plastic and Reconstructive Surgery. Global Open·Christian A FoyDamien P Kuffler
Jan 22, 2022·Journal of Materials Science. Materials in Medicine·Antonio MerolliLauren Bright

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