Effect of locally infused IGF-I on femoral gene expression and bone turnover activity in old rats

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
A WakisakaC T Liang

Abstract

Previously, we showed that the age-dependent deficit in bone formation activity can be attributed in part to a decline in local expression of insulin-like growth factor I (IGF-I) and altered mitogenic response of old osteoprogenitor cells to IGF-I. To establish the cellular basis for using IGF-I as a possible therapeutic agent for osteoporosis, we examined the effect of locally infused (50 ng/day for 14 days) on the expression of osteoblast-related genes in femurs of old rats. Northern and dot blot analyses showed that the expression of procollagen (I), osteopontin, alkaline phosphatase, and osteocalcin was increased 0.4- to 1.5-fold in IGF-I-treated femurs as compared with control femurs. Histomorphometric analyses were carried out in parallel experiments to assess the changes in bone remodeling activity. Trabecular bone volume, trabecular number, and trabecular thickness were increased 56%, 29%, and 23%, respectively, whereas trabecular separation was reduced 26% by IGF-I treatment. IGF-I treatment increased significantly the osteoid volume, osteoid surface, osteoblast number, and osteoblast surface. Mineralizing surface and mineral apposition rate, kinetic indices of bone formation, were also stimulated by IGF-I treatment. T...Continue Reading

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Citations

Apr 14, 2007·International Orthopaedics·Aziz AbbaspourNatsuo Yasui
Nov 9, 2000·The Journal of Clinical Investigation·N A SimsP A Kelly
Aug 31, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John L FowlkesCharles K Lumpkin
Apr 23, 2003·Muscle & Nerve·Magali LouisMarc Francaux
Apr 8, 2016·Physiology·Jawed A Siddiqui, Nicola C Partridge
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T T AndreassenH Oxlund
May 28, 2009·Clinical Orthopaedics and Related Research·Yener N YeniDavid P Fyhrie
May 10, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jay J CaoBernard P Halloran
Nov 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Klara SjögrenClaes Ohlsson
May 24, 2014·Clinical Implant Dentistry and Related Research·Akira KumasakaTakashi Sasano
Oct 6, 2001·Biochemical and Biophysical Research Communications·H TanakaC T Liang
Jan 14, 2000·Kidney International. Supplement·J A Hoyland, M L Picton

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