Effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (Pagrus major) sperm

Cryobiology
Ya Kun ChenDao Yuan Ma

Abstract

The aim of this study was to determine the effect of long-term cryopreservation on physiological characteristics, the antioxidant activities and lipid peroxidation of red seabream sperm which were respectively cryopreserved with 15% dimethylsulfoxide (Me(2)SO) for 1 month, 13 months, 26 months, 48 months and 73 months. The motility and fertility of post-thaw sperm decreased with the storage time going on. The highest motility (87.67±2.52%) was obtained in sperm cryopreserved for 1 month and the lowest (50.67±5.31%) was in sperm for 73 months. There were no significant differences (p<0.05) in fertilization rates of sperm cryopreserved for 1 month (71.33±8.84%), 13 months (69.22±1.02%) and 26 months (60.33±2.33%); however, the sperm fertility decreased significantly for 48 months (47.22±3.89%) and 73 months (39.56±0.69%) storage. In addition, superoxide dismutase (SOD) activities of sperm were at a stable level for less than 26 months storage, then, decreased significantly after 48 months storage. Catalase (CAT) activities of sperm cryopreserved for 13 months, 26 months, 48 months and 73 months were significantly lower than that for 1 month. There were no significant differences in the malondialdehyde (MDA) level of sperm for les...Continue Reading

References

Jan 1, 1993·Reproduction, Fertility, and Development·W M Maxwell, S Salamon
Feb 5, 2000·Molecular Reproduction and Development·J F BilodeauC Gagnon
Aug 5, 2000·Animal Reproduction Science·W V Holt
Sep 8, 2000·American Journal of Veterinary Research·B A BallI K Liu
Oct 11, 2002·Theriogenology·Jin-Chywan GwoHsien-Yu Cheng
Mar 24, 2004·Theriogenology·Shuyang HeL Curry Woods
May 26, 2004·Cryobiology·Christina WaltersPhillip C Stanwood
Sep 23, 2008·Animal Reproduction Science·Shuyan DingYahong Huang

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Citations

Aug 15, 2012·Ecotoxicology and Environmental Safety·Adele FabbrociniGiovanni Sansone
Aug 26, 2014·Reproductive Biology·Viktoriya Dzyuba, Jacky Cosson
Mar 29, 2020·Animal Reproduction Science·Marco Aurélio Schiavo NovaesCiro Alexandre Alves Torres

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