Effect of long-term partial bladder outlet obstruction on caldesmon isoforms and their correlation with contractile function

Acta Pharmacologica Sinica
Lin YangXin-Yang Wang

Abstract

In the present study, we investigate the expression of caldesmon (CAD) isoforms in rabbit detrusor smooth muscles (DSM) during the progression of partial bladder outlet obstruction and relate them with the time course of obstruction. Detrusor samples were obtained from the bladders of rabbits with partial bladder outlet obstruction and sham-operated control rabbits after 1, 2, 4, and 8 weeks of obstruction. Contractile responses to field stimulation and carbachol were determined in the isolated bladder strips. Western blotting was used to determine the relative levels of CaD isoform expression at the protein levels. The contractile responses decreased progressively over the course of obstruction. The expression of l-CaD increased significantly to approximately the same extent as the 1-4-week obstructed groups and further in the 8-week obstructed group. The expression of h-CaD increased in all of the obstructed bladders, but at significantly higher levels in the 1-2-week obstructed bladders compared to the control and 4-8-week obstructed bladders. The changes in the isoforms of CaD may be part of the molecular mechanism for bladder compensation following partial bladder outlet obstruction. The overexpression of l-CaD and the h-C...Continue Reading

References

Oct 1, 1991·The Biochemical Journal·S B Marston, C S Redwood
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K SobueS Kakiuchi
Jan 1, 1994·World Journal of Urology·S Chacko, P A Longhurst
Oct 8, 1999·Molecular Biology of the Cell·D M HelfmanA D Bershadsky
Oct 26, 1999·Journal of Muscle Research and Cell Motility·J R Haeberle
Feb 15, 2001·The Journal of Urology·A SchröderR M Levin
Nov 17, 2004·Scandinavian Journal of Urology and Nephrology. Supplementum·Samuel ChackoAlan Wein

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Citations

Mar 9, 2011·Biomechanics and Modeling in Mechanobiology·Rebecca L HeiseMichael S Sacks
Feb 27, 2013·The Journal of Sexual Medicine·Maureen E BashaSamuel Chacko
Sep 17, 2013·Neurourology and Urodynamics·Martin HennenbergChristian Gratzke
Apr 24, 2010·American Journal of Obstetrics and Gynecology·Peter TakacsCarlos A Medina
Apr 8, 2009·International Review of Cell and Molecular Biology·Jim Jung-Ching LinJian-Ping Jin
Nov 6, 2009·Nature Reviews. Urology·Karen J Aitken, Darius J Bägli

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