Effect of loss of T-DNA genes on MIA biosynthetic pathway gene regulation and alkaloid accumulation in Catharanthus roseus hairy roots.

Plant Cell Reports
Jyoti TanejaAlok Krishna Sinha

Abstract

Hairy roots are generated by integration of T-DNA in host plant genome from root inducing (Ri) plasmid of Agrobacterium rhizogenes and have been utilized for production of secondary metabolites in different plant systems. In Catharanthus roseus, hairy roots are known to show different morphologies, growth patterns, and alkaloid contents. It is also known that during transformation, there is a differential loss of a few T-DNA genes. To decipher the effect of loss of T-DNA genes on the various aspects of hairy roots, ten hairy root clones were analyzed for the presence or absence of T-DNA genes and its implications. It was found that the loss of a few ORFs drastically affects the growth and morphological patterns of hairy roots. The absence of T(R)-DNA from hairy roots revealed increased transcript accumulation and higher alkaloid concentrations, whereas callusing among hairy root lines led to decreased transcript and alkaloid accumulation. Significantly higher expression of MIA biosynthetic pathway genes and low abundance of regulator transcripts in hairy root clones in comparison with non-transformed control roots were also observed. This study indicates that it is not only the integration of T-DNA at certain region of host pla...Continue Reading

References

Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Jan 1, 1984·Journal of Bacteriology·G A HuffmanE W Nester
Aug 16, 2000·Journal of Experimental Botany·R D FirnJ Digby
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·L Otten, A Helfer
Jun 14, 2002·Journal of Chromatography. a·C Tikhomiroff, M Jolicoeur
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Marie UmberLéon Otten
Oct 2, 2002·Molecular Biotechnology·Felipe Vázquez-FlotaMaria de Lourdes Miranda-Ham
Nov 30, 2002·Trends in Pharmacological Sciences·Pascal Gantet, Johan Memelink
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Marie UmberLéon Otten
Jun 19, 2007·Phytochemistry·Mary MagnottaVincenzo De Luca
Apr 25, 2008·Biotechnology Advances·Victor P Bulgakov
Mar 5, 1993·Biotechnology and Bioengineering·R BhadraJ V Shanks
Feb 1, 1989·Plant Cell Reports·M H BrillanceauJ Tempé
Aug 1, 1988·Plant Cell Reports·A J ParrM J Rhodes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.