Abstract
We recently described the 'spontaneous' migration of skin dendritic cells out of human split skin during culture. Since newly infiltrating cells from the circulation are excluded, this in vitro model is very suitable for studying the effect of UVB irradiation on the migratory properties, phenotype and functional capacities of skin cells. In the present study, we show that UVB irradiation of the skin before the culture period results in a significantly lower number of migrated cells that could be obtained compared with untreated skin. Relatively more dendritic cells of the population that migrated from UVB-irradiated skin were of dermal origin, as indicated by a higher percentage of CD1b+ cells. These data imply that UVB irradiation inhibits migration, especially of the epidermal Langerhans cells. Ultrastructural analysis of the irradiated skin revealed that the UVB dose used did not cause any directly visible damage to the cells. However, the cell population that had migrated from UVB-irradiated skin showed a significantly lower capacity to stimulate allogeneic T cells. This was not due to a lower expression of MHC class II on these cells. The percentage of cells expressing B7.1, B7.2 and LFA-3 was decreased in the population m...Continue Reading
Citations
Jan 19, 2011·Immunology and Cell Biology·Heather M McGeeGregory M Woods
Oct 1, 1996·Experimental Dermatology·M B LappinM Norval
Dec 19, 1998·Experimental Dermatology·N YawalkarL R Braathen
Apr 1, 1997·Photochemistry and Photobiology·P A SteerenbergH Van Loveren
Jan 18, 2002·Infection and Immunity·Heather JebbariStella C Knight
Jun 1, 1997·Photodermatology, Photoimmunology & Photomedicine·J ViacA Claudy
Nov 5, 1997·The Journal of Investigative Dermatology·I B KremerM B Teunissen
Nov 12, 2010·The Journal of Investigative Dermatology·Krista OuwehandSusan Gibbs
May 25, 2006·Photodermatology, Photoimmunology & Photomedicine·Motoko HamakawaTakeshi Horio
Oct 3, 1999·The Journal of Investigative Dermatology·K TakayamaK Nishioka
Sep 18, 2004·The Journal of Investigative Dermatology·Rainer Hofmann-WellenhofPeter Wolf
Dec 18, 1998·The Journal of Investigative Dermatology·S BacciJ W Streilein
Aug 4, 1998·FEBS Letters·S RobinsonH Griffiths
Apr 1, 1997·European Journal of Immunology·J K Laihia, C T Jansen
Apr 1, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Salvador GonzalezNeena Philips
Oct 30, 2019·Medical Physics·Samuel W RemediosDzung L Pham
Dec 30, 2014·Archives of Dermatological Research·Olga María Palomino