Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells

Lasers in Medical Science
Ivana Maria ZaccaraCarlos Augusto Galvão Barboza

Abstract

A positive effect of low-level laser irradiation (LLLI) on the proliferation of some cell types has been observed, but little is known about its effect on dental pulp stem cells (DPSCs). The aim of this study was to identify the lowest energy density able to promote the proliferation of DPSCs and to maintain cell viability. Human DPSCs were isolated from two healthy third molars. In the third passage, the cells were irradiated or not (control) with an InGaAlP diode laser at 0 and 48 h using two different energy densities (0.5 and 1.0 J/cm²). Cell proliferation and viability and mitochondrial activity were evaluated at intervals of 24, 48, 72, and 96 h after the first laser application. Apoptosis- and cell cycle-related events were analyzed by flow cytometry. The group irradiated with an energy density of 1.0 J/cm² exhibited an increase of cell proliferation, with a statistically significant difference (p < 0.05) compared to the control group at 72 and 96 h. No significant changes in cell viability were observed throughout the experiment. The distribution of cells in the cell cycle phases was consistent with proliferating cells in all three groups. We concluded that LLLI, particularly a dose of 1.0 J/cm², contributed to the grow...Continue Reading

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Citations

Nov 1, 2016·Photomedicine and Laser Surgery·Márcia Martins MarquesMaria Stella Moreira
May 31, 2016·International Endodontic Journal·A P TurrioniJ Hebling
Aug 1, 2016·Journal of Photochemistry and Photobiology. B, Biology·Ali Borzabadi-Farahani
Nov 2, 2017·Einstein·Fernanda GinaniCarlos Augusto Galvão Barboza
Aug 29, 2019·Brazilian Oral Research·Ivana Maria ZaccaraPatrícia Maria Poli Kopper
Oct 14, 2017·Lasers in Medical Science·Fernanda GinaniCarlos Augusto Galvão Barboza
Jun 24, 2020·Lasers in Medical Science·Sachin KulkarniRoy George
Feb 6, 2020·Journal of Lasers in Medical Sciences·Malihe MirhosseiniShirin Farivar
Jan 12, 2021·Journal of Lasers in Medical Sciences·Jéssica Meirinhos MirandaMarleny Elizabeth Marquez de Martinez Gerbi
Feb 23, 2021·Aesthetic Plastic Surgery·Hernán PintoElena Sánchez-Vizcaíno Mengual
Apr 23, 2021·Lasers in Medical Science·Na LiuChongtao Zhu
Apr 24, 2021·Journal of the World Federation of Orthodontists·Chaiyapol ChaweewannakornRochaya Chintavalakorn

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