Effect of low molecular weight proteins and dextran on renal cathepsin B and L activity

Kidney International
C J OlbrichtC C Tisher

Abstract

Renal extraction of low molecular weight proteins (LMWP) accounts for 30% to 80% of their total metabolic clearance. Extraction includes glomerular filtration, proximal tubular uptake, and intralysosomal proteolysis. To characterize the anatomic sites and enzymes involved in digestion of reabsorbed LMWP, the lysosomal proteases, cathepsin B and L, were measured by ultramicroassay in isolated S1, S2 and S3 segments of the proximal tubule of proteinuric rats. Increased glomerular filtration and tubular uptake of LMWP were induced by i.v. and i.p. injections of myoglobin and cationic and anionic lysozyme. Both cationic lysozyme and myoglobin increased cathepsin B and L activities in the proximal tubule, while anionic lysozyme had no effect. Morphologic examination of kidney tissue suggested that proximal tubular uptake of anionic lysozyme was negligible in comparison with the cationic form. Hence, only LMWP absorbed by the proximal tubule cells stimulated cathepsin B and L activities. Proximal tubular uptake of cationic lysozyme was determined by measurement of lysozyme activities in S1, S2, and S3. S1 segments contained the highest lysozyme activity, while S2 and S3 had much lower activities, and cathepsin B and L activity follow...Continue Reading

References

Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H G Rennke, M A Venkatachalam
Sep 1, 1979·Kidney International·E I Christensen, A B Maunsbach
Jul 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·G LITWACK

Citations

Jun 23, 2016·Chemico-biological Interactions·Ljubava D ZorovaDmitry B Zorov
May 2, 2003·American Journal of Physiology. Renal Physiology·Jakub GburekErik I Christensen
Jan 25, 2013·American Journal of Physiology. Renal Physiology·D LeeD A Power

Related Concepts

Ctsl protein, rat
Metazoa
Cathepsin B-Like Proteinase
Cathepsins
Cysteine Endopeptidases
Saviosol
Glomerular Filtration Rate
Kidney Tubules, Proximal
Leftose
Myoglobin

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