Effect of low temperature on stability of theta-type plasmids in Carnobacterium maltaromaticum

FEMS Microbiology Letters
Valerie M BohaychukLynn M McMullen

Abstract

The heterologous production of useful peptides such as bacteriocins by lactic acid bacteria (LAB) has been studied for use in the biopreservation of foods. Recombinant plasmids can suffer drawbacks such as segregational instability affecting the production of these peptides in certain environments such as absence of selective pressure or low temperature. The link between growth temperature characteristics of parental strains and stability of theta-type plasmids at a low temperature was investigated. The growth of four parental strains at 4 degrees C and stability of five derivative theta-type plasmids transformed into Carnobacterium maltaromaticum UAL26 at 25 and 4 degrees C were determined. Two plasmids (pCD11 and pCaT) derived from psychrotrophic LAB and plasmid, pHW800, from Enterococcus faecium 226 with unknown growth temperature characteristics, had excellent stability when strains were grown at 4 degrees C. Plasmids (pTRKH2 and pUCB820) derived from LAB that did not grow at refrigeration temperatures were not stable at 4 degrees C. When a DNA fragment from pCD11 containing 22-bp repeats, a putative replication initiation site, and the gene for the RepA protein was inserted into pTRKH2, the resulting derivative plasmid was...Continue Reading

References

Oct 1, 1995·Applied and Environmental Microbiology·M J van Belkum, M E Stiles
Jun 1, 1995·Journal of Bacteriology·R W WoroboM E Stiles
Dec 1, 1995·International Journal of Food Microbiology·T AbeeC Hill
Nov 1, 1996·International Journal of Food Microbiology·E BorchY Blixt
Nov 14, 1997·Applied Microbiology and Biotechnology·J ArnauH Israelsen
Mar 21, 1998·Applied and Environmental Microbiology·N HornH M Dodd
Nov 13, 1998·FEMS Microbiology Letters·L AxelssonA Holck
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·P Langella, Y Le Loir
Aug 5, 1999·Letters in Applied Microbiology·J K McCormickM E Stiles
Jan 25, 2000·Applied Microbiology and Biotechnology·K DuanN W Dunn
Mar 2, 2002·Applied and Environmental Microbiology·Marc VancanneytWilhelm H Holzapfel
Aug 14, 2002·Journal of Applied Microbiology·J MetaxopoulosE H Drosinos
Oct 17, 2002·International Journal of Food Microbiology·J M RodríguezH M Dodd
Oct 31, 2002·Applied and Environmental Microbiology·B HenrichU Wegmann
Nov 22, 2002·Trends in Biotechnology·Jos F M L Seegers
Jun 6, 2003·Applied and Environmental Microbiology·Yoshikatsu HamasakiHidetoshi Morita
Jun 12, 2004·The Journal of Dairy Research·Antonio FernándezJuan M Rodríguez
Feb 1, 1993·Applied and Environmental Microbiology·R KiewietS Bron
Oct 1, 1993·Molecular Microbiology·Rense KiewietJos F M L Seegers

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Citations

Dec 1, 2012·Probiotics and Antimicrobial Proteins·Marco J van Belkum, John C Vederas
Jun 21, 2019·Applied Microbiology and Biotechnology·Vitor Troccoli RibeiroEveraldo Silvino Dos Santos

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