PMID: 7033902Nov 1, 1981Paper

Effect of lysosomotropic amine, chloroquine, on the radioactive incorporation pattern of glycosaminoglycans in human skin fibroblasts

Pathologie-biologie
M Moczar

Abstract

Control and chloroquine treated fibroblast cultures were incubated with 14C-glucosamine. The cells were separated from the medium and the pericellular matrix. The S-carboxymethylproteins in the medium were hydrolysed with trypsin and pronase. The glycosaminoglycans were treated with hyaluronidase, chondroitin AB-lyase and chondroitin ABC-lyase. The macromolecular 14C label per cell in the medium was higher in the chloroquine treated cultures than in the controls. The incorporation of the 14C-hexosamines per cell into the pericellular and intracellular compartment was not modified. The increased 14C-label in the medium of fibroblast exposed to chloroquine accounted for the increased incorporation of the 14C precursor into chondroitin 4 and 6 sulfates and dermatan sulfate.

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