Effect of macrophages on breast cancer cell proliferation, and on expression of hormone receptors, uPAR and HER-2

International Journal of Oncology
Therése LindstenAnn Erlandsson

Abstract

Malignant tumors, including breast cancers, are frequently infiltrated with innate immune cells and tumor-associated macrophages (TAMs) represent the major inflammatory component in stroma of many tumors. In this study, we examined the immunoreactivity of the macrophage markers CD68 and CD163 as well as the hormone receptors estrogen receptor α (ERα), progesterone receptor (PR), estrogen receptor β1 (ERβ1), human epidermal growth factor receptor 2 (HER-2), matrix metalloproteinase 9 (MMP‑9), urokinase-type plasminogen activator receptor (uPAR) and the proliferations marker Ki67 in 17 breast cancer biopsies. The quantitative score for CD68+ and CD163+ strongly indicate M2 phenotype dominance in the currently investigated biopsies. We found that an increasing level of macrophages was negatively associated with ERα or PR, whereas a positive association was observed for Ki-67 or uPAR. No significant association could be seen between the level of macrophage and HER-2, ERβ1 or MMP-9 expression. Effect of conditioned media (CM) generated from cultured human M1 and M2 macrophage phenotypes were investigated on the proliferation and expression of selected markers in the T47D breast cancer cell line. We found that in contrast to the in v...Continue Reading

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Citations

Feb 23, 2018·Breast Cancer Research and Treatment·Yoon Jin ChaJa Seung Koo
Mar 21, 2020·International Journal of Cancer. Journal International Du Cancer·Martina RaudenskaMichal Masarik
Aug 19, 2020·Cellular Oncology (Dordrecht)·Cansu TekinC Arnold Spek
Jun 26, 2020·International Journal of Molecular Sciences·Hye Min Kim, Ja Seung Koo
Apr 4, 2019·Journal of breast cancer·Hasong JeongSun Young Kwon
Oct 21, 2019·Journal of Biomedical Science·Yibing ChenZhengzhi Zou
Nov 24, 2020·BioMed Research International·Xingling QiGuiling Li
Feb 11, 2021·BioMed Research International·Jianjun LuYingling Zhou

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Methods Mentioned

BETA
FCS
PCR
biopsies

Software Mentioned

LinRegPCR
ModFit LT

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