Effect of magnesium ions on fermentative and respirative functions in Pichia stipitis under oxygen-restricted growth

Microbiological Research
G Mahler, C Nudel

Abstract

Mg2+ level affected growth, xylitol and ethanol production by P. stipitis grown under microaerophilic conditions. Low Mg2+ level (1 mM) directed the C flux from ethanol to xylitol, with no effect on xylose consumption rate. The addition of pyrazole, an alcohol dehydrogenase (ADH) inhibitor, had the same effect, even in conditions of Mg2+ excess (4 mM), indicating a negative interaction between ADH and Mg2+ ions (p < 0.01). Cells grown either with pyrazole or Mg limitation increased their intracellular NADH concentration about 3 times, but displayed no significant differences in ADH specific activities (1,000 U/mg protein, +/- 10%). In contrast, no interaction was measured between Mg and antimycin A, excluding the possibility that Mg2+ limitation interferes with respiration.

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Terasaki, H Rubin
Jan 1, 1985·Antonie van Leeuwenhoek·T LodiI Ferrero
Jun 1, 1973·Analytical Biochemistry·C Bernofsky, M Swan
Dec 10, 1980·Molecular and Cellular Biochemistry·S Y CechM E Maguire
Apr 1, 1983·Journal of General Microbiology·P M BruinenbergW A Scheffers
Jan 1, 1997·Journal of Industrial Microbiology & Biotechnology·G M Walker, A I Maynard
Jan 1, 1983·Biotechnology and Bioengineering·C S GongG T Tsao

Citations

Apr 24, 2008·Biotechnology Letters·Frank K Agbogbo, Guillermo Coward-Kelly
Feb 22, 2012·Molecular Biotechnology·Li LuluHong Jiong
Jul 13, 2016·Waste Management·M S Romero-GüizaS Astals
Oct 4, 2000·Yeast
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny
May 25, 2021·Food Chemistry·Normando Ribeiro-FilhoIan D Fisk

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