Effect of magnetic nanoparticles of Fe3O4 and 5-bromotetrandrine on reversal of multidrug resistance in K562/A02 leukemic cells.

International Journal of Nanomedicine
Jian ChengXuemei Wang

Abstract

This study aims to evaluate the multidrug resistance (MDR) reversal activity by magnetic nanoparticles of Fe3O4 (MNPs-Fe3O4) and 5-bromotetrandrine (BrTet) MDR cell line K562/A02 solitarily or symphysially. The proliferation of K562 and K562/A02 cells and the cytotoxicity on peripheral blood mononuclear cells (PMBCs) were evaluated by MTT assay. Cellular accumulation of daunorubicin (DNR) was analyzed by flow cytometry. Real-time polymerase chain reaction and Western blotting analyses were performed to examine the mRNA and protein levels of mdr1, respectively. The results showed that the combination of MNPs-Fe3O4 and BrTet with effective concentrations significantly increased cytotoxicity against MDR cell line K562/A02. Both BrTet and MNPs-Fe3O4 increased the intracellular DNR accumulation in the K562/A02 cell line, and downregulated the level of mdr1 gene and expression of P-glycoprotein. Furthermore, the combination did not have significant cytotoxicity in PMBCs. We propose that MNPs-Fe3O4 conjugated with DNR and BrTet probably have synergetic effects on MDR reversal.

References

Dec 11, 2003·Cancer Chemotherapy and Pharmacology·Liwu FuQichao Pan
Sep 21, 2004·Cancer Chemotherapy and Pharmacology·Jing JinGengtao Liu
Oct 21, 2004·Best Practice & Research. Clinical Haematology·Douglas D Ross
Jun 17, 2006·Pharmaceutical Research·Patrick Couvreur, Christine Vauthier
Jan 30, 2008·CA: a Cancer Journal for Clinicians·Xu WangDong M Shin

❮ Previous
Next ❯

Citations

Dec 21, 2010·International Journal of Nanomedicine·Weiwei WuJieyong Li
Aug 31, 2013·International Journal of Nanomedicine·Akiko SatoMasatoshi Watanabe

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

Statistical Package for Social Science

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.